Effect of dietary additives on intestinal permeability in both Drosophila and a human cell co-culture.
Effect of dietary additives on intestinal permeability in both Drosophila and a human cell co-culture.
复制标题
膳食添加剂对果蝇和人类细胞共培养物肠道通透性的影响。
DOI：
10.1242/dmm.034520
复制
发表时间：
2018-11-28
影响因子：
4.3
通讯作者：
Musselman LP
中科院分区：
文献类型：
--
作者：
Pereira MT;Malik M;Nostro JA;Mahler GJ;Musselman LP
肠道屏障通透性的增加与衰老和疾病有关，包括2型糖尿病、克罗恩病、乳糜泻、多发性硬化症和肠易激综合征。随着工业食品加工和食品添加剂消费的增加，这些疾病的发病率也在上升。包括糖、金属氧化物纳米颗粒、表面活性剂和氯化钠在内的添加剂都被认为可以增加肠道通透性。我们使用了两个互补的模型系统来研究食品添加剂对肠道屏障功能的影响：果蝇体内模型和体外人类细胞共培养模型。在测试的添加剂中，高糖的肠道通透性增加最为显著。高糖也增加了摄食，但减少了肠道和动物的整体尺寸。我们还研究了食品添加剂如何影响肠道黏膜防御因子肠道碱性磷酸酶（IAP）的活性，该因子随细菌负荷而波动，并影响肠道通透性。我们发现高糖降低了两种模型的IAP活性。在两种模型中，人工操纵微生物组影响肠道通透性，揭示了两者之间的复杂关系。这项研究扩展了之前对果蝇和人类的研究，表明饮食可以在肠道屏障的健康中发挥作用。此外，可以使用简单的模型来研究饮食对肠道通透性和功能影响的机制。摘要：糖和其他食品添加剂可以抑制人类细胞共培养和黑腹果蝇的肠道屏障功能。
Increased intestinal barrier permeability has been correlated with aging and disease, including type 2 diabetes, Crohn's disease, celiac disease, multiple sclerosis and irritable bowel syndrome. The prevalence of these ailments has risen together with an increase in industrial food processing and food additive consumption. Additives, including sugar, metal oxide nanoparticles, surfactants and sodium chloride, have all been suggested to increase intestinal permeability. We used two complementary model systems to examine the effects of food additives on gut barrier function: a Drosophila in vivo model and an in vitro human cell co-culture model. Of the additives tested, intestinal permeability was increased most dramatically by high sugar. High sugar also increased feeding but reduced gut and overall animal size. We also examined how food additives affected the activity of a gut mucosal defense factor, intestinal alkaline phosphatase (IAP), which fluctuates with bacterial load and affects intestinal permeability. We found that high sugar reduced IAP activity in both models. Artificial manipulation of the microbiome influenced gut permeability in both models, revealing a complex relationship between the two. This study extends previous work in flies and humans showing that diet can play a role in the health of the gut barrier. Moreover, simple models can be used to study mechanisms underlying the effects of diet on gut permeability and function. Summary: Sugar and other food additives can inhibit gut barrier function in both human cell co-cultures and Drosophila melanogaster.
登录
查看更多内容
通过肠道碱性磷酸酶预防小鼠中与抗生素相关的代谢综合征。
DOI：
10.1111/dom.12645
发表时间：
2016-05
期刊：
Diabetes, obesity & metabolism
影响因子：
--
作者：
Economopoulos KP;Ward NL;Phillips CD;Teshager A;Patel P;Mohamed MM;Hakimian S;Cox SB;Ahmed R;Moaven O;Kaliannan K;Alam SN;Haller JF;Goldstein AM;Bhan AK;Malo MS;Hodin RA
通讯作者：
Hodin RA
影响因子：
8.8
作者：
Clark RI;Salazar A;Yamada R;Fitz-Gibbon S;Morselli M;Alcaraz J;Rana A;Rera M;Pellegrini M;Ja WW;Walker DW
通讯作者：
Walker DW
影响因子：
4.6
作者：
Dambroise E;Monnier L;Ruisheng L;Aguilaniu H;Joly JS;Tricoire H;Rera M
通讯作者：
Rera M
影响因子：
4.8
作者：
Barone, Sharon;Fussell, Stacey L.;Soleimani, Manoocher
通讯作者：
Soleimani, Manoocher
影响因子：
4.1
作者：
Deshpande, Gaurang;Mapanga, Rudo F.;Essop, M. Faadiel
通讯作者：
Essop, M. Faadiel