In vivo assessment for oral delivery of Bacillus subtilis harboring a viral protein (VP28) against white spot syndrome virus in Litopenaeus vannamei
In vivo assessment for oral delivery of Bacillus subtilis harboring a viral protein (VP28) against white spot syndrome virus in Litopenaeus vannamei
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体内评估含有病毒蛋白（VP28）的枯草芽孢杆菌针对凡纳滨对虾白斑综合症病毒的口服递送
DOI：
10.1016/j.aquaculture.2011.09.036
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发表时间：
2011-12
期刊：
影响因子：
4.5
通讯作者：
Fu linglin
中科院分区：
文献类型：
--
作者：
Fu linglin
枯草芽孢杆菌芽胞作为益生菌被食用，作为表达和口服抗原蛋白的活载体已被探索。在我们的初步实验中，通过口服携带VP28的枯草杆菌芽胞(rVP28-bs)给凡纳滨对虾，可以观察到在白斑综合征病毒(WSSV)攻击下的极高的存活率(相对存活率：83.3%)。接种rVP28-bs后，检测血细胞吞噬功能和免疫相关基因表达水平。经rVP28-bs处理的对虾，其血细胞吞噬白斑综合征病毒的百分率(P&0.001)显著高于对照组(P<0.05)。而对虾非亲缘病毒TSV的吞噬百分率，各试验组间差异不显著(p>；0.05)。这表明对虾在rVP28-bs“免疫”后的高吞噬活性和高水平存活是对WSSV具有选择性或特异性的。此外，与对照组相比，rVP28-bs组和B.subtilis组免疫相关基因(proPO、PE和LGBP)均显著上调(p<；0.05)，但rVP28-bs组和B.subtilis组之间差异不显著(p>；0.05)。结果表明，rVP28-bs增强的吞噬功能是保护对虾免受病毒侵染的重要机制，而rVP28-bs刺激的体液反应仅在对虾的抗病毒防御中起辅助作用。此外，rVP28-bs孢子的体内存活和扩散分析表明，作为一种健壮的生命形式，孢子可以通过肠道存活，萌发表达rVP28并发挥益生菌作用，并在血淋巴中传播，在排泄之前将VP28呈递给对虾防御系统。这些结果可能为该重组枯草杆菌芽胞在对虾养殖场抗WSSV感染中的应用前景提供参考。
Bacillus subtilis spores, being consumed as probiotics, have been explored as live carriers for expression and oral delivery of antigen proteins. In our initial experiment, by oral delivery of B. subtilis spores harboring VP28 (rVP28-bs) to Litopenaeus vannamei, the extremely high survival (Relative Percent Survival: 83.3%) upon challenge with white spot syndrome virus (WSSV) can be observed. After ‘vaccination’ with rVP28-bs, the hemocytic phagocytosis and immune-related gene expression levels in hemocytes were analyzed. The percentage of haemocytes phagocytosing WSSV was significantly higher (p<0.001) in shrimp previously fed with rVP28-bs (48.2±6.3) than the controls (11.0±3.5 and 8.1±2.5). However, there were no significant differences (p>0.05) in all the experimental groups for the percentage phagocytosis of TSV (an unrelated virus of shrimp). This suggests that the heightened phagocytic activity, and thus the high-level survival of shrimp after rVP28-bs ‘vaccination’ are selective or specific towards WSSV. Moreover, immune-related genes (proPO, PE and LGBP) were significantly (p<0.05) upregulated in both rVP28-bs and B. subtilis feeding groups compared to the control, though no significant differences (p>0.05) were observed between rVP28-bs and B. subtilis groups. It was indicated that the phagocytosis enhanced by rVP28-bs was the essential one to protect shrimp from virus infection, while the rVP28-bs-stimulated humoral response only plays an assistant role in antiviral defense of shrimp. Besides, in vivo fate and dissemination assays of rVP28-bs spores showed that, as robust life forms, spores can survive transit across the gut tract, germinate to express rVP28 and exert probiotic action, and disseminate in the haemolymph to present VP28 to the shrimp defense system before being excreted. These results may raise the application prospective of this recombinant B. subtilis spores against WSSV infection in shrimp farms.
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影响因子：
2.9
作者：
Chou, Pin-Hsiang;Chang, Hao-Shuo;Wang, K. C. Han-Ching
通讯作者：
Wang, K. C. Han-Ching
DOI：
10.1002/prot.10640
发表时间：
2004-05
期刊：
Proteins: Structure
影响因子：
--
作者：
Xiaobo Zhang;C. Huang;Xu-hua Tang;Ying Zhuang;C. Hew
通讯作者：
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影响因子：
9.8
作者：
Dong, Yuemei;Taylor, Harry E;Dimopoulos, George
通讯作者：
Dimopoulos, George
影响因子：
4.5
作者：
Du, Huahua;Xu, Zirong;Dai, Wei
通讯作者：
Dai, Wei
影响因子：
5.4
作者：
Sritunyalucksana, Kallaya;Wannapapho, Wanphen;Flegel, Timothy W.
通讯作者：
Flegel, Timothy W.