Effect of oral administration of metronidazole or prednisolone on fecal microbiota in dogs.
Effect of oral administration of metronidazole or prednisolone on fecal microbiota in dogs.
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DOI：
10.1371/journal.pone.0107909
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tsujimoto H
中科院分区：
文献类型：
--
作者：
Igarashi H;Maeda S;Ohno K;Horigome A;Odamaki T;Tsujimoto H
胃肠道微生物群与狗的各种胃肠道疾病的发病机制有关，包括急性腹泻和慢性肠病。甲硝唑和强的松龙通常用于治疗这些疾病；然而，它们对胃肠道微生物群的影响尚未被研究。本研究的目的是评估这些药物对狗的胃肠道微生物群的影响。甲硝唑以12.5 mg/kg的剂量每天两次给5只健康狗，强的松龙以1.0 mg/kg的剂量每天给另一组5只健康狗，持续14天。在给药前后（第0天和第14天）以及停药后14天和28天（第28天和第42天）采集粪便样本。提取DNA，利用新一代测序技术（Illumina MiSeq）基于16S核糖体RNA （rRNA）基因序列测定每个样品的细菌多样性和组成。甲硝唑组细菌多样性指数在第14天显著下降，停药后恢复。基于未加权和加权UniFrac距离矩阵的主坐标分析和分层树形图构建显示，与其他时间点相比，甲硝唑在第14天也显著改变了细菌组成。Bacteroidaceae、Clostridiaceae、Fusobacteriaceae、Lachnospiraceae、Ruminococcaceae、Turicibacteraceae和Veillonellaceae的比例下降，而bifidobacteraceae、Enterobacteriaceae、Enterococcaceae和Streptococcaceae的比例在第14天增加，到第42天恢复到初始比例。相反，强的松龙对细菌多样性和组成没有影响。通过施用甲硝唑减少梭杆菌等致病菌和增加双歧杆菌等有益菌可能有利于促进胃肠道健康；然而，需要进一步调查对患病犬的影响。
Gastrointestinal microbiota have been implicated in the pathogenesis of various gastrointestinal disorders in dogs, including acute diarrhea and chronic enteropathy. Metronidazole and prednisolone are commonly prescribed for the treatment of these diseases; however, their effects on gastrointestinal microbiota have not been investigated. The objective of this study was to evaluate the effects of these drugs on the gastrointestinal microbiota of dogs. Metronidazole was administered twice daily at 12.5 mg/kg to a group of five healthy dogs, and prednisolone at 1.0 mg/kg daily to a second group of five healthy dogs for 14 days. Fecal samples were collected before and after administration (day 0 and 14), and 14 and 28 days after cessation (day 28 and 42). DNA was extracted, and the bacterial diversity and composition of each sample were determined based on 16S ribosomal RNA (rRNA) gene sequences using next-generation sequencing (Illumina MiSeq). In the group administered metronidazole, bacterial diversity indices significantly decreased at day 14, and recovered after the cessation. Principal coordinates analysis and hierarchical dendrogram construction based on unweighted and weighted UniFrac distance matrices revealed that bacterial composition was also significantly altered by metronidazole at day 14 compared with the other time points. The proportions of Bacteroidaceae, Clostridiaceae, Fusobacteriaceae, Lachnospiraceae, Ruminococcaceae, Turicibacteraceae, and Veillonellaceae decreased, while Bifidobacteriaceae, Enterobacteriaceae, Enterococcaceae, and Streptococcaceae increased at day 14 and returned to their initial proportions by day 42. Conversely, no effect of prednisolone was observed on either the bacterial diversity or composition. Reducing pathogenic bacteria such as Fusobacteria and increasing beneficial bacteria such as Bifidobacterium through the administration of metronidazole may be beneficial for promoting gastrointestinal health; however, further investigations into the effects on diseased dogs are needed.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
9.4
作者：
De La Cochetière, MF;Durand, T;Doré, J
通讯作者：
Doré, J
影响因子：
14.9
作者：
Claesson MJ;Wang Q;O'Sullivan O;Greene-Diniz R;Cole JR;Ross RP;O'Toole PW
通讯作者：
O'Toole PW
影响因子：
4.2
作者：
Beloshapka, Alison N.;Dowd, Scot E.;Swanson, Kelly S.
通讯作者：
Swanson, Kelly S.
影响因子：
11.8
作者：
BORRIELLO, SP
通讯作者：
BORRIELLO, SP