The influence of age on insecticide susceptibility of Anopheles arabiensis during dry and rainy seasons in rice irrigation schemes of Northern Tanzania.
The influence of age on insecticide susceptibility of Anopheles arabiensis during dry and rainy seasons in rice irrigation schemes of Northern Tanzania.
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DOI：
10.1186/s12936-017-2022-6
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发表时间：
2017-09-11
期刊：
影响因子：
3
通讯作者：
Kweka EJ
中科院分区：
文献类型：
--
作者：
Mbepera S;Nkwengulila G;Peter R;Mausa EA;Mahande AM;Coetzee M;Kweka EJ
杀虫剂耐药性是坦桑尼亚疟疾病媒控制规划面临的主要新挑战。适当的监测和发现对指导病媒控制规划至关重要。本文研究了坦桑尼亚北部干旱和雨季阿拉伯按蚊种群衰老对杀虫剂抗性状况的影响。在旱季和雨季分别从稻田取样冈比亚按蚊幼虫，并将其饲养至成虫。分别于2、3、5、10日龄雏鸟分别接触溴氰菊酯、氯菊酯、高效氯氰菊酯、滴滴涕、苯虫威和甲基吡虫磷6种杀虫剂，观察龄期对雏鸟抗药性的影响。使用世卫组织标准药敏试验试剂盒使蚊子接触杀虫剂。暴露1 h时记录敲除，24 h后记录死亡率和耐药率。蚊虫标本采用聚合酶链反应（PCR）法进行种级鉴定。326份标本经PCR鉴定为阿拉伯按蚊323份（99.1%）。在旱季和雨季，从幼虫饲养的成虫暴露于所有拟除虫菊酯和苯虫威时，死亡率降低（从61%到97.7%不等），而它们对滴滴涕和吡虫磷-甲基完全敏感。枯水期和雨季接触拟除虫菊酯类杀虫剂后，蚊子的死亡率均随年龄的增加而显著增加（DF = 1, P < 0.05）。拟除虫菊酯暴露后，雨季蚊虫死亡率显著高于旱季（DF = 1, P < 0.05）。与拟除虫菊酯类杀虫剂相比，本虫威暴露于不同年龄和季节的蚊虫死亡率均较高（94.0% ~ 99.8%）。甲基吡啶磷仅在雨季进行了试验，因此无法与旱季蚊子进行比较。结果表明，安。在两个季节，Arabiensis对拟除虫菊酯类杀虫剂都有抗性，并且年轻年龄组的抗性水平高于老年年龄组。不论季节和年龄，蚊子对滴滴涕和甲基吡虫磷完全敏感。
Insecticide resistance is the major emerging challenge facing the malaria vector control programmes in Tanzania. Proper monitoring and detection is of paramount importance guiding the vector control programmes. This paper presents the effect of mosquito aging on insecticide resistance status in Anopheles arabiensis populations in dry and rainy seasons in northern Tanzania. Anopheles gambiae s.l. larvae were sampled from rice fields in both dry and rainy seasons and reared in the insectary to adults. The emerged females in batches of 2, 3, 5, and 10 days old were exposed to six insecticides (deltamethrin, permethrin, lambda-cyhalothrin, DDT, bendiocarb and pirimiphos-methyl) to see the effects of age on insecticide resistance. Mosquitoes were exposed to insecticides using WHO standard susceptibility test kits. Knockdown was recorded during the 1-h exposure, while mortality and resistance ratio were recorded 24 h later. Mosquito specimens were identified to species level using the polymerase chain reaction (PCR) method. Among the 326 specimens processed by PCR, 323 (99.1%) were identified as Anopheles arabiensis. There was reduced mortality (ranging from 61 to 97.7%) when adults reared from larvae were exposed to all pyrethroids and bendiocarb in both dry and rainy seasons, while they were fully susceptible to DDT and pirimiphos-methyl. There was a significant increase in mortality rate with increase in mosquito’s age in both dry and rainy seasons following exposure to pyrethroids (DF = 1, P < 0.05). Mosquitoes showed significantly higher mortality rates in the rainy season than in the dry season after being exposed to pyrethroids (DF = 1, P < 0.05). Higher mortality rates (94.0–99.8%) were observed in all ages and seasons when mosquitoes were exposed to bendiocarb compared with pyrethroids. Pirimiphos-methyl was only tested in the rainy season so no comparison with dry season mosquitoes could be made. Results showed that An. arabiensis were resistant to pyrethroids in both seasons and that the young age groups exhibited higher levels of resistance compared with the older age groups. Mosquitoes were full susceptible to DDT and pirimiphos-methyl irrespective of the season and age.
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相对于单独的方法，相对于单独的两种方法，对长期杀虫剂处理的网和室内残留喷涂的组合的比较现场评估，在主载体是Anopheles Aropheles Arabiensis的区域中预防疟疾。
影响因子：
3.2
作者：
Okumu FO;Mbeyela E;Lingamba G;Moore J;Ntamatungiro AJ;Kavishe DR;Kenward MG;Turner E;Lorenz LM;Moore SJ
通讯作者：
Moore SJ
影响因子：
3.2
作者：
Choi, Kwang S.;Christian, Riann;Coetzee, Maureen
通讯作者：
Coetzee, Maureen
DOI：
10.1016/j.trstmh.2011.01.009
发表时间：
2011-05-01
影响因子：
2.2
作者：
Rajatileka, Shavanthi;Burhani, Joseph;Ranson, Hilary
通讯作者：
Ranson, Hilary
影响因子：
3
作者：
Bonner, Kimberly;Mwita, Alex;Brown, Nick
通讯作者：
Brown, Nick
影响因子：
1.9
作者：
Hunt, RH;Brooke, BD;Coetzee, M
通讯作者：
Coetzee, M