Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice.
Germinal center architecture disturbance during Plasmodium berghei ANKA infection in CBA mice.
复制标题
CBA小鼠中质质体的生发中心结构障碍。
DOI：
10.1186/1475-2875-6-59
复制
发表时间：
2007-05-16
期刊：
影响因子：
3
通讯作者：
Lenzi, Henrique L.
中科院分区：
文献类型：
--
作者：
Carvalho, Leonardo J. M.;Ferreira-da-Cruz, Maria F.;Daniel-Ribeiro, Claudio T.;Pelajo-Machado, Marcelo;Lenzi, Henrique L.
对疟疾血液期感染的免疫反应一般是有缺陷的，需要长期暴露在寄生虫中才能获得免疫，而且在许多情况下随着免疫病理状态的发展，如脑型疟疾。难以形成保护性免疫的潜在原因之一是记忆反应发育不良。本文探讨了伯氏疟原虫感染CBA小鼠时，淋巴器官(脾、淋巴结和Peyer‘s结)的细胞反应性与免疫和病理的关系。用1×106伯氏疟原虫寄生红细胞感染CBA小鼠，分别于感染后第3、6~8、10天处死小鼠。取脾、淋巴结和Peyer‘s斑块，用Carson’s福尔马林固定，切成5μm切片，放入玻片中，用Lennert‘s Giemsa和苏木精-伊红染色，在明视场显微镜下观察。感染早期(第3天)次级淋巴器官内T细胞有较强的活化，感染后第6~8天，B细胞大量激活，失去常规生发中心结构，中心母细胞激活、增殖和凋亡增强，但向中心细胞分化较少。脾脏边缘带消失，生发中心与红髓的界限模糊。在T细胞区观察到强烈的浆细胞生成。观察到的变化，尤其是中心细胞分化不良的生发中心结构障碍(GCAD)，表明小鼠感染伯氏疟原虫时B细胞反应存在缺陷，可能影响B细胞的记忆反应。
Immune responses to malaria blood stage infection are in general defective, with the need for long-term exposure to the parasite to achieve immunity, and with the development of immunopathology states such as cerebral malaria in many cases. One of the potential reasons for the difficulty in developing protective immunity is the poor development of memory responses. In this paper, the potential association of cellular reactivity in lymphoid organs (spleen, lymph nodes and Peyer's patches) with immunity and pathology was evaluated during Plasmodium berghei ANKA infection in CBA mice. CBA mice were infected with 1 × 106 P. berghei ANKA-parasitized erythrocytes and killed on days 3, 6–8 and 10 of infection. The spleen, lymph nodes and Peyer's patches were collected, fixed in Carson's formalin, cut in 5 μm sections, mounted in glass slides, stained with Lennert's Giemsa and haematoxylin-eosin and analysed with bright-field microscopy. Early (day 3) strong activation of T cells in secondary lymphoid organs was observed and, on days 6–8 of infection, there was overwhelming activation of B cells, with loss of conventional germinal center architecture, intense centroblast activation, proliferation and apoptosis but little differentiation to centrocytes. In the spleen, the marginal zone disappeared and the limits between the disorganized germinal center and the red pulp were blurred. Intense plasmacytogenesis was observed in the T cell zone. The observed alterations, especially the germinal center architecture disturbance (GCAD) with poor centrocyte differentiation, suggest that B cell responses during P. berghei ANKA infection in mice are defective, with potential impact on B cell memory responses.
登录
查看更多内容
影响因子：
2.2
作者：
Sanchez-Torres, L;Rodriguez-Ropon, A;Favila-Castillo, L
通讯作者：
Favila-Castillo, L
影响因子：
3.1
作者：
Helmby, H;Jönsson, G;Troye-Blomberg, M
通讯作者：
Troye-Blomberg, M
影响因子：
--
作者：
Baird, JK
通讯作者：
Baird, JK
影响因子：
2
作者：
Riccio, EKP;Neves, I;Ferreira-da-Cruz, MD
通讯作者：
Ferreira-da-Cruz, MD
影响因子：
4.4
作者：
Achtman, AH;Khan, M;Langhorne, J
通讯作者：
Langhorne, J