Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway.
Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway.
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Chs1p 和 Chs3p 是参与几丁质合成的两种蛋白质，位于酿酒酵母内吞途径的一个区室中。
DOI：
10.1091/mbc.7.12.1909
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发表时间：
1996
影响因子：
3.3
通讯作者：
Schekman,RW
中科院分区：
文献类型：
--
作者：
Ziman,M;Chuang,JS;Schekman,RW
在酿酒酵母中，几丁质（一种细胞壁多糖）的合成在细胞周期和形态发生方面受到时间和空间调节。使用免疫试剂，我们发现两种几丁质合酶 Chs1p 和 Chs3p 的稳态水平在细胞周期中没有波动，表明它们不仅仅受到合成和降解的调节。先前的细胞分级分离研究表明，几丁质合酶 I 活性 (CSI) 以质膜形式和称为壳质体的细胞内膜结合颗粒存在。壳质体被提议充当储存库，用于调节几丁质合酶向分裂隔膜的运输。我们发现 Chs1p 和 Chs3p 部分存在于壳质中，并且这种分布不受细胞周期调节。脉冲追踪细胞分级分离实验表明，在内吞作用突变体 (end4-1) 中，壳质体的产生被阻断，表明内吞作用对于壳质体的形成或维持是必需的。此外，Ste2p通过配体诱导的内吞作用内化，并与壳质体共分级，表明这些膜蛋白填充在相同的内体区室中。然而，与 Ste2p 相比，Chs1p 和 Chs3p 没有快速降解，因此提出了几丁质合成的时间和空间调节是通过内体几丁质合酶池的动员介导的可能性。
In Saccharomyces cerevisiae, the synthesis of chitin, a cell-wall polysaccharide, is temporally and spatially regulated with respect to the cell cycle and morphogenesis. Using immunological reagents, we found that steady-state levels of Chs1p and Chs3p, two chitin synthase enzymes, did not fluctuate during the cell cycle, indicating that they are not simply regulated by synthesis and degradation. Previous cell fractionation studies demonstrated that chitin synthase I activity (CSI) exists in a plasma membrane form and in intracellular membrane-bound particles called chitosomes. Chitosomes were proposed to act as a reservoir for regulated transport of chitin synthase enzymes to the division septum. We found that Chs1p and Chs3p resided partly in chitosomes and that this distribution was not cell cycle regulated. Pulse-chase cell fractionation experiments showed that chitosome production was blocked in an endocytosis mutant (end4-1), indicating that endocytosis is required for the formation or maintenance of chitosomes. Additionally, Ste2p, internalized by ligand-induced endocytosis, cofractionated with chitosomes, suggesting that these membrane proteins populate the same endosomal compartment. However, in contrast to Ste2p, Chs1p and Chs3p were not rapidly degraded, thus raising the possibility that the temporal and spatial regulation of chitin synthesis is mediated by the mobilization of an endosomal pool of chitin synthase enzymes.
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影响因子：
56.9
作者：
Jos é Ruiz;Salomon Bartnicki
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/0005-2736(88)90408-7
发表时间：
1988
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
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通讯作者：
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DOI：
--
发表时间：
1982
期刊：
影响因子：
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作者：
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通讯作者：
P. Novick
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影响因子：
7.8
作者：
N. Davis;J. Horecka;G. Sprague
通讯作者：
G. Sprague