BRUTUS and its paralogs, BTS LIKE1 and BTS LIKE2, encode important negative regulators of the iron deficiency response in Arabidopsis thaliana.
BRUTUS and its paralogs, BTS LIKE1 and BTS LIKE2, encode important negative regulators of the iron deficiency response in Arabidopsis thaliana.
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DOI：
10.1039/c7mt00152e
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发表时间：
2017-07-19
期刊：
影响因子：
--
通讯作者：
Guerinot ML
中科院分区：
文献类型：
--
作者：
Hindt MN;Akmakjian GZ;Pivarski KL;Punshon T;Baxter I;Salt DE;Guerinot ML
铁是植物健康所必需的，但过量的铁也可能是有毒的。因此，必须严格控制铁的水平。拟南芥E3连接酶Brutus(BTS)参与缺铁反应的负调控，我们发现拟南芥的两个同源基因BTS Like 1(BTSL1)和BTS LIKE2(BTSL2)编码冗余的蛋白质，作为缺铁反应的负调节因子。这两种E3连接酶的缺失增强了对铁缺乏的耐受性。我们进一步产生了一个三重突变体，失去了BTS的两个同源基因和部分BTS的表达，表现出对缺铁条件的更强的耐受性，增加了铁的积累，而不会产生任何铁毒效应。最后，我们鉴定了一个携带一种新的BTS错义突变的突变体，该突变体在缺铁和缺铁条件下生长时在根中都表现出缺铁反应，导致植物在缺铁条件下生长时产生铁毒。
Iron (Fe) is required for plant health, but it can also be toxic when present in excess. Therefore, Fe levels must be tightly controlled. The Arabidopsis thaliana E3 ligase BRUTUS (BTS) is involved in the negative regulation of the Fe deficiency response and we show here that the two A. thaliana BTS paralogs, BTS LIKE1 (BTSL1) and BTS LIKE2 (BTSL2) encode proteins that act redundantly as negative regulators of the Fe deficiency response. Loss of both of these E3 ligases enhances tolerance to Fe deficiency. We further generated a triple mutant with loss of both BTS paralogs and a partial loss of BTS expression that exhibits even greater tolerance to Fe-deficient conditions and increased Fe accumulation without any resulting Fe toxicity effects. Finally, we identified a mutant carrying a novel missense mutation of BTS that exhibits an Fe deficiency response in the root when grown under both Fe-deficient and Fe-sufficient conditions, leading to Fe toxicity when plants are grown under Fe-sufficient conditions.
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影响因子：
16.6
作者：
Kobayashi, Takanori;Nagasaka, Seiji;Senoura, Takeshi;Itai, Reiko Nakanishi;Nakanishi, Hiromi;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.
影响因子：
56.9
作者：
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通讯作者：
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影响因子：
7.4
作者：
Li, Xiaoli;Zhang, Huimin;Yu, Diqiu
通讯作者：
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影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
5.1
作者：
Korshunova, YO;Eide, D;Pakrasi, HB
通讯作者：
Pakrasi, HB