Establishment of a versatile cell line for juvenile hormone signaling analysis in Tribolium castaneum.
Establishment of a versatile cell line for juvenile hormone signaling analysis in Tribolium castaneum.
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DOI：
10.1038/srep01570
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发表时间：
2013
影响因子：
4.6
通讯作者：
Shinoda, Tetsuro
中科院分区：
文献类型：
--
作者：
Kayukawa, Takumi;Tateishi, Ken;Shinoda, Tetsuro
赤粉甲虫已被广泛用作基因功能分析的实验室模型。在本研究中，我们建立了一个新的细胞系(Tc81)，并通过分析保幼激素(JH)信号通路，验证了该细胞系的实用性。在Tc81细胞中，依赖于JH的昆虫变态抑制因子Krüppel Homolog 1(Kr-H1)在亚纳摩尔水平的JHS作用下被迅速诱导。生物信息学分析和报告分析发现2个JH反应元件(KJHREs)位于转录起始点上游区域和Kr-H1的第一内含子中。此外，耐甲氧丁二烯(Met)和类固醇受体共激活剂(SRC)的RNAi降低了JH依赖的Kr-H1转录本的诱导和kJHRE报告基因的活性。因此，这个新的Tc81细胞系对于阐明JH信号是有用的，并且是一个很有前途的RNAi和报告分析基因功能分析的工具。
The red flour beetle, Tribolium castaneum, has been widely used as a laboratory model for analyzing gene function. In this study, we established a novel cell line (Tc81) from T. castaneum embryos and validated the utility of this cell line by analyzing the juvenile hormone (JH) signaling pathway. In Tc81 cells, the Krüppel homolog 1 gene (Kr-h1), which is a JH-dependent repressor of insect metamorphosis, was rapidly induced by subnanomolar levels of JHs. Bioinformatics analysis and reporter assays identified 2 JH response elements (kJHREs) located in the region upstream of the transcription start site and in the first intron of Kr-h1. Furthermore, methoprene tolerant (Met) and steroid receptor co-activator (SRC) RNAi reduced JH-dependent induction of Kr-h1 transcripts and kJHRE-reporter activities. Thus, this novel Tc81 cell line is useful for the elucidation of JH signaling and is a promising tool for the functional analysis of genes by RNAi and reporter assays.
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影响因子：
3.5
作者：
Bitra, Kavita;Tan, Anjiang;Dowling, Ashley;Palli, Subba R.
通讯作者：
Palli, Subba R.
DOI：
10.1073/pnas.1116123109
发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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