Isolation of deer tick virus (Powassan virus, lineage II) from Ixodes scapularis and detection of antibody in vertebrate hosts sampled in the Hudson Valley, New York State.
Isolation of deer tick virus (Powassan virus, lineage II) from Ixodes scapularis and detection of antibody in vertebrate hosts sampled in the Hudson Valley, New York State.
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DOI：
10.1186/1756-3305-6-185
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发表时间：
2013-07-15
影响因子：
3.2
通讯作者：
Kramer LD
中科院分区：
文献类型：
--
作者：
Dupuis AP 2nd;Peters RJ;Prusinski MA;Falco RC;Ostfeld RS;Kramer LD
鹿蜱病毒（Deer tick virus，DTV）是波瓦桑病毒（Powassan virus，POWV）的一个遗传学和生态学上不同的谱系，也称为谱系II POWV。在过去的15年里，由于DTV的出现，特别是在纽约州的哈德逊山谷，战俘脑炎的人类发病率增加了。我们发起了一个广泛的采样活动，以确定是否POWV是现存的整个哈德逊谷蜱媒和/或脊椎动物宿主。从宿主或植被中收集了13，000多只蜱，并使用分子和病毒分离技术检测了DTV的存在。将肩胛硬蜱（黑腿蜱）的脊椎动物宿主诱捕（哺乳动物）或网住（鸟类），并分析血液样品中是否存在POWV中和抗体。计算最大似然估计值（MLE），以确定每个研究中心蜱的感染率。从2007年到2012年，每年都在若虫和成虫I中发现DTV的证据。从哈德逊河谷采集的肩胛骨58个蜱池的病毒和/或RNA呈阳性。从哈德逊河以东地区收集的成年蜱的感染率较高。在检测到DTV的地点，MLE限值范围为0.2-6.0感染成人/100。弗吉尼亚负鼠、条纹臭鼬和浣熊是收集的感染若虫蜱的来源。在土拨鼠（4/6）、负鼠（1/6）和鸟类（4/727）中检测到POWV感染的血清学证据。血统I，原型POWV，没有被检测到。这些数据表明，DTV在整个哈德逊河谷，特别是河以东地区广泛的地方性传播。在最近发现POW脑炎病例的县中检测到高感染率，支持谱系II POWV，DTV负责这些人类感染的假设。
Deer tick virus, DTV, is a genetically and ecologically distinct lineage of Powassan virus (POWV) also known as lineage II POWV. Human incidence of POW encephalitis has increased in the last 15 years potentially due to the emergence of DTV, particularly in the Hudson Valley of New York State. We initiated an extensive sampling campaign to determine whether POWV was extant throughout the Hudson Valley in tick vectors and/or vertebrate hosts. More than 13,000 ticks were collected from hosts or vegetation and tested for the presence of DTV using molecular and virus isolation techniques. Vertebrate hosts of Ixodes scapularis (black-legged tick) were trapped (mammals) or netted (birds) and blood samples analyzed for the presence of neutralizing antibodies to POWV. Maximum likelihood estimates (MLE) were calculated to determine infection rates in ticks at each study site. Evidence of DTV was identified each year from 2007 to 2012, in nymphal and adult I. scapularis collected from the Hudson Valley. 58 tick pools were positive for virus and/or RNA. Infection rates were higher in adult ticks collected from areas east of the Hudson River. MLE limits ranged from 0.2-6.0 infected adults per 100 at sites where DTV was detected. Virginia opossums, striped skunks and raccoons were the source of infected nymphal ticks collected as replete larvae. Serologic evidence of POWV infection was detected in woodchucks (4/6), an opossum (1/6), and birds (4/727). Lineage I, prototype POWV, was not detected. These data demonstrate widespread enzootic transmission of DTV throughout the Hudson Valley, in particular areas east of the river. High infection rates were detected in counties where recent POW encephalitis cases have been identified, supporting the hypothesis that lineage II POWV, DTV, is responsible for these human infections.
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DOI：
10.1007/bf01923553
发表时间：
1993-09-15
期刊：
EXPERIENTIA
影响因子：
--
作者：
LABUDA, M;NUTTALL, PA;SABO, A
通讯作者：
SABO, A
影响因子：
3.7
作者：
Grard, Gilda;Moureau, Gregory;de Lamballerie, Xavier
通讯作者：
de Lamballerie, Xavier
DOI：
10.1073/pnas.0233733100
发表时间：
2003-01-21
影响因子：
11.1
作者：
LoGiudice, K;Ostfeld, RS;Keesing, F
通讯作者：
Keesing, F
影响因子：
11.8
作者：
Birge J;Sonnesyn S
通讯作者：
Sonnesyn S
DOI：
10.4269/ajtmh.2004.71.3.0700268
发表时间：
2004-09-01
影响因子：
3.3
作者：
Ebel, GD;Kramer, LD
通讯作者：
Kramer, LD