In Vitro Antimicrobial Activity of Diacerein on 76 Isolates of Gram-Positive Cocci from Bacterial Keratitis Patients and In Vivo Study of Diacerein Eye Drops on Staphylococcus aureus Keratitis in Mice
In Vitro Antimicrobial Activity of Diacerein on 76 Isolates of Gram-Positive Cocci from Bacterial Keratitis Patients and In Vivo Study of Diacerein Eye Drops on Staphylococcus aureus Keratitis in Mice
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双醋瑞因对76株细菌性角膜炎患者革兰氏阳性球菌的体外抗菌活性及双醋瑞因滴眼液对小鼠金黄色葡萄球菌角膜炎的体内研究
DOI：
10.1128/aac.01874-18
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发表时间：
2019-02
影响因子：
4.9
通讯作者：
Wang Liya
中科院分区：
文献类型：
--
作者：
Zhang Hongmin;Liu Susu;Yue Juan;Sun Shengtao;Lv Qixue;Jian Shoujun;Xie Yanting;Han Lei;Zhang Fenfen;Dai Yanfang;Wang Liya
细菌性角膜炎是一种侵袭性的感染性角膜疾病。随着抗生素耐药性的持续上升以及新抗生素发现的减少，现在需要新的抗菌药物。摘要：细菌性角膜炎是一种侵袭性的……
Bacterial keratitis is an aggressive infectious corneal disease. With the continuing rise in antibiotic resistance and a decline in the discovery of new antibiotics, new antimicrobial drugs are now required. ABSTRACT Bacterial keratitis is an aggressive infectious corneal disease. With the continuing rise in antibiotic resistance and a decline in the discovery of new antibiotics, new antimicrobial drugs are now required. In the present study, we determined the antibacterial activity of diacerein, an anti-inflammatory drug, against 76 Gram-positive cocci isolated from bacterial keratitis patients in vitro and anti-Staphylococcus aureus activity in a mouse bacterial keratitis model in vivo. The MICs of diacerein were tested using the broth microdilution method in vitro. A BALB/c Staphylococcus aureus keratitis animal model was selected and the corneal clinical observation, viable bacteria, and hematoxylin-eosin and Gram staining of infected corneas were measured to evaluate the antibacterial efficacy of diacerein eye drops in vivo. An in vivo eye irritation study was carried out by a modified Draize test in rabbits. Our in vitro results showed that diacerein possesses satisfactory antibacterial activity against the majority of Gram-positive cocci (60/76), including all 57 tested Staphylococcus spp. and 3 Enterococcus spp. The in vivo experiment showed that diacerein eye drops reduced bacterial load and improved ocular clinical scores after topical administration of diacerein drops on infected corneas. The ocular irritation test revealed that diacerein eye drop had excellent ocular tolerance. These results indicated that diacerein possesses in vivo anti-Staphylococcus aureus activity. We suggest that diacerein is a possible topically administered drug for Staphylococcus aureus-infected patients, especially those with ocular surface inflammatory disorders.
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影响因子：
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作者：
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