Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis.
Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis.
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半滑舌鳎睾丸细胞系的建立和表征
DOI：
10.7150/ijbs.7.452
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发表时间：
2011-04-15
影响因子：
9.2
通讯作者：
Chen SL
中科院分区：
文献类型：
--
作者：
Zhang B;Wang X;Sha Z;Yang C;Liu S;Wang N;Chen SL
成年人睾丸内精子发生是研究干细胞更新和分化的一个极好的系统，它受睾丸体细胞的控制。为了研究半滑舌鳎（Cynoglossus semilaevis）的精子发生过程，我们从半滑舌鳎幼鱼性腺中建立了一个细胞系CSGC。CSGC由成纤维细胞样细胞组成，保留42条染色体的二倍体核型，缺乏异配子W染色体，缺乏雌性特异性标记，并表达睾丸体细胞的标记dmrt。因此，CSGC似乎由睾丸体细胞组成。我们表明，该细胞系是有效的感染大菱鲆红体病虹彩病毒和比目鱼淋巴囊肿病病毒的细胞病变效应和病毒的繁殖在病毒感染的细胞中的外观证明，最令人信服的是，通过电子显微镜观察病毒颗粒，demonstrating CSGC是适合于研究病毒和宿主细胞之间的相互作用。CSGC作为ZZ-ZW遗传性别决定系统中的第一个鱼类睾丸体细胞系，为研究精子发生过程中体细胞与生殖细胞之间的相互作用及性别相关事件提供了有用的工具。
Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to understanding spermatogenesis in the half-smooth tongue sole (Cynoglossus semilaevis) as a marine fish model of aquaculture importance, we established a cell line called CSGC from a juvenile gonad of this organism. CSGC is composed of fibroblast-like cells, retains a diploid karyotype of 42 chromosomes, lacks the heterogametic W chromosome, lacks a female specific marker and expresses the dmrt, a marker for testicular somatic cells. Therefore, CSGC appears to consist of testicular somatic cell cells. We show that this cell line is effective for infection by the turbot reddish body iridovirus and flounder lymphocystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electon microscopy, demonstrateing that CSGC is suitable to study interactions between virus and host cells. As a first fish testicular somatic cell line of the ZZ-ZW genetic sex determination system, CSGC will be a useful tool to study sex-related events and interactions between somatic cells and germ cells during spermatogenesis.
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影响因子：
4.5
作者：
Chen, SL;Ren, GC;Hong, YH
通讯作者：
Hong, YH
影响因子：
3
作者：
Chen, Song-Lin;Li, Jing;Xu, Jian-Yong
通讯作者：
Xu, Jian-Yong
影响因子：
4.4
作者：
Brunner, B;Hornung, U;Schartl, M
通讯作者：
Schartl, M
影响因子：
3.9
作者：
Lutfalla, G;Roest Crollius, H;Robinson-Rechavi, M
通讯作者：
Robinson-Rechavi, M
DOI：
10.1073/pnas.88.13.5774
发表时间：
1991-07-01
影响因子：
11.1
作者：
MIURA, T;YAMAUCHI, K;NAGAHAMA, Y
通讯作者：
NAGAHAMA, Y