Densovirus is a mutualistic symbiont of a global crop pest (Helicoverpa armigera) and protects against a baculovirus and Bt biopesticide.
Densovirus is a mutualistic symbiont of a global crop pest (Helicoverpa armigera) and protects against a baculovirus and Bt biopesticide.
复制标题
DOI：
10.1371/journal.ppat.1004490
复制
发表时间：
2014-10
期刊：
影响因子：
6.7
通讯作者：
Wu K
中科院分区：
文献类型：
--
作者：
Xu P;Liu Y;Graham RI;Wilson K;Wu K
共生细菌和它们的宿主之间的互惠关系在昆虫系统中很常见。然而，病毒通常被认为是病原体，即使有些病毒被报道对其宿主有益。在此，我们报告了一种新的浓核病毒，棉铃虫浓核病毒-1（HaDNV-1），似乎是有益的，它的主机。HaDNV-1广泛存在于H.棉铃虫成虫（2008年至2012年期间流行率>67%）。HaDNV-1与H.棉铃虫核型多角体病毒（HaNPV），一种广泛用作生物农药的杆状病毒。实验室生物测定显示，HaDNV-1宿主幼虫对HaNPV的抗性显著增强（病毒载量较低），对苏云金芽孢杆菌（Bt）毒素的抗性在低剂量下也较高。实验室检测表明，病毒主要分布在脂肪体中，可以水平传播和垂直传播，但前者只发生在大的挑战剂量。浓核病毒阳性个体比未感染的昆虫发育更快，繁殖力更高。我们没有发现HaDNV-1感染对H.棉铃虫适合度相关的性状，强烈表明棉铃虫和其浓核病毒之间的互利相互作用。棉铃虫Helicoverpa armigera是亚洲、欧洲、非洲和澳大利亚最重要的农作物害虫之一。在此，我们报告了一种新的浓核病毒（HaDNV-1），它广泛分布在野生群体的H。HaDNV-1对棉铃虫有明显的增效作用，对棉铃虫幼虫和蛹的发育速率、雌虫寿命和生殖力均有明显的促进作用，提示HaDNV-1与棉铃虫之间存在互利互作。棉铃虫是目前广泛控制的生物农药苏云金芽孢杆菌（Bt）毒素和杆状病毒HaNPV。因此，重要的是要估计共生病毒对这些生物农药的效率产生负面影响的风险。田间和实验室结果表明，HaDNV-1感染显着增加幼虫对HaNPV和Bt毒素的抗性。这些结果对该物种生物农药的选择具有重要意义，并强调需要对可能影响宿主个体和种群动态的优雅微生物相互作用进行更多研究。
Mutualistic associations between symbiotic bacteria and their hosts are common within insect systems. However, viruses are often considered as pathogens even though some have been reported to be beneficial to their hosts. Herein, we report a novel densovirus, Helicoverpa armigera densovirus-1 (HaDNV-1) that appears to be beneficial to its host. HaDNV-1 was found to be widespread in wild populations of H. armigera adults (>67% prevalence between 2008 and 2012). In wild larval populations, there was a clear negative interaction between HaDNV-1 and H. armigera nucleopolyhedrovirus (HaNPV), a baculovirus that is widely used as a biopesticide. Laboratory bioassays revealed that larvae hosting HaDNV-1 had significantly enhanced resistance to HaNPV (and lower viral loads), and that resistance to Bacillus thuringiensis (Bt) toxin was also higher at low doses. Laboratory assays indicated that the virus was mainly distributed in the fat body, and could be both horizontally- and vertically-transmitted, though the former occurred only at large challenge doses. Densovirus-positive individuals developed more quickly and had higher fecundity than uninfected insects. We found no evidence for a negative effect of HaDNV-1 infection on H. armigera fitness-related traits, strongly suggesting a mutualistic interaction between the cotton bollworm and its densovirus. The old world cotton bollworm, Helicoverpa armigera, is one of the most significant pests of crops throughout Asia, Europe, Africa and Australia. Herein, we report a novel densovirus (HaDNV-1) which was widely distributed in wild populations of H. armigera and was beneficial to its host by increasing larval and pupal development rates, female lifespan and fecundity, suggesting a mutualistic interaction between the cotton bollworm and HaDNV-1. The cotton bollworm is currently widely controlled by the biopesticides Bacillus thuringiensis (Bt) toxin and the baculovirus HaNPV. It is therefore important to estimate the risk that the symbiotic virus will negatively impact on the efficiency of these biopesticides. Field and laboratory results suggest that HaDNV-1 infection significantly increases larval resistance to HaNPV and Bt toxin. These results have important implications for the selection of biopesticides for this species, and highlight the need for greater research into the elegant microbial interactions that may impact host individual and population dynamics.
登录
查看更多内容
DOI：
10.1111/j.1749-6632.1975.tb35099.x
发表时间：
1975-01-01
影响因子：
5.2
作者：
FINE, PEM
通讯作者：
FINE, PEM
影响因子：
2.2
作者：
Feng, HQ;Wu, KM;Guo, YY
通讯作者：
Guo, YY
影响因子：
3.4
作者：
Chen, Yanping;Evans, Jay;Feldlaufer, Mark
通讯作者：
Feldlaufer, Mark
影响因子：
4.4
作者：
Gunning, RV;Dang, HT;Moores, GD
通讯作者：
Moores, GD
影响因子：
3.1
作者：
Feng, Hongqiang;Gould, Fred;Wu, Kongming
通讯作者：
Wu, Kongming