Effect of the environment on the dendritic morphology of the rat auditory cortex.
Effect of the environment on the dendritic morphology of the rat auditory cortex.
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DOI：
10.1002/syn.20710
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发表时间：
2010-02
期刊：
影响因子：
2.3
通讯作者：
Atzori, Marco
中科院分区：
文献类型：
--
作者：
Bose, Mitali;Munoz-Llancao, Pablo;Roychowdhury, Swagata;Nichols, Justin A.;Jakkamsetti, Vikram;Porter, Benjamin;Byrapureddy, Rajasekhar;Salgado, Humberto;Kilgard, Michael P.;Aboitiz, Francisco;Dagnino-Subiabre, Alexies;Atzori, Marco
本研究旨在确定环境诱导的初级听觉皮层（A1）兴奋性突触变化的形态学相关。我们使用高尔基-考克斯染色技术比较了暴露于不同环境操作的Sprague-Dawley大鼠锥体细胞的树突特性。使用耳分析、树突长度测量和脊柱密度计数来监测感觉耳聋和听觉版环境富集（EE）的影响。我们发现，耳聋减少了树突的顶端长度，而基底树突长度不变，而EE选择性地增加了基底树突长度，而顶端树突长度不变。相反，耳聋降低，而EE增加了A1层2/3 （LII/III）神经元基底和根尖树突的脊柱密度。为了确定应激是否导致了A1的形态学变化，我们在缺乏行为相关声信号的约束诱导模型中研究了神经形态学。我们发现，压力选择性地减少了听觉初级皮层的树突长度，而不是视觉初级皮层。与声学操作相似，应力诱导的树突长度变化具有层特异性模式，应激动物的LII/III神经元具有正常的根尖树突，但基部树突较短，而颗粒内神经元（V层和VI层）具有较短的根尖树突，但基部树突正常。同样的处理并没有在视觉皮层中引起类似的变化，这表明听觉皮层是新皮层可塑性的一个非常敏感的目标，并且长时间暴露于不同的声音和情绪环境操纵可能会产生特定的树突形状和脊柱密度变化。
The present study aimed to identify morphological correlates of environment-induced changes at excitatory synapses of the primary auditory cortex (A1). We used the Golgi-Cox stain technique to compare pyramidal cells dendritic properties of Sprague-Dawley rats exposed to different environmental manipulations. Sholl analysis, dendritic length measures, and spine density counts were used to monitor the effects of sensory deafness and an auditory version of environmental enrichment (EE). We found that deafness decreased apical dendritic length leaving basal dendritic length unchanged, whereas EE selectively increased basal dendritic length without changing apical dendritic length. On the contrary, deafness decreased while EE increased spine density in both basal and apical dendrites of A1 layer 2/3 (LII/III) neurons. To determine whether stress contributed to the observed morphological changes in A1, we studied neural morphology in a restraint-induced model that lacked behaviorally relevant acoustic cues. We found that stress selectively decreased apical dendritic length in the auditory but not in the visual primary cortex. Similar to the acoustic manipulation, stress-induced changes in dendritic length possessed a layer specific pattern displaying LII/III neurons from stressed animals with normal apical dendrites but shorter basal dendrites, while infragranular neurons (layers V and VI) displayed shorter apical dendrites but normal basal dendrites. The same treatment did not induce similar changes in the visual cortex, demonstrating that the auditory cortex is an exquisitely sensitive target of neocortical plasticity, and that prolonged exposure to different acoustic as well as emotional environmental manipulation may produce specific changes in dendritic shape and spine density.
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