Identification of 24h Ixodes scapularis immunogenic tick saliva proteins.
Identification of 24h Ixodes scapularis immunogenic tick saliva proteins.
复制标题
DOI：
10.1016/j.ttbdis.2015.03.012
复制
发表时间：
2015-04
影响因子：
3.2
通讯作者：
Mulenga A
中科院分区：
文献类型：
--
作者：
Lewis LA;Radulović ŽM;Kim TK;Porter LM;Mulenga A
肩部硬蜱可以说是美国医学上最重要的扁虱物种。这种扁虱传播了美国14种人类壁虱传播疾病(TBD)病原体中的5种：伯氏疏螺旋体、吞噬细胞无浆菌、宫本巴贝斯虫、微小巴贝斯虫和波瓦桑病毒病。除鲍瓦桑病毒病外，以肩周肌为媒介的待定病毒剂需要在附着后24小时以上才能传播。本研究鉴定了24小时免疫原性的扁虱唾液蛋白，这可能为制定在大多数病原体传播之前停止取食扁虱的策略提供机会。用兔抗24 h饲养的肩袖吸虫雌蜱唾液蛋白的抗体，构建了24 h饲养的雌性肩蜂噬菌体展示基因表达文库，并进行了下一代测序、重新组装和生物信息学分析。共组装了182个重叠群，其中~19%(35/182)是新发现的，与GenBank中的任何已知蛋白质都没有同源性。剩余的~81%(147/182)的重叠群是根据GenBank中的匹配暂时确定的，其中~18%(27/147)与先前被注释为假想和推定的扁虱唾液蛋白的蛋白质序列相匹配。其他还包括蛋白水解酶和蛋白水解酶抑制剂(~3%，5/147)、转运蛋白和/或配体结合蛋白(~6%，9/147)、免疫原性硬蜱唾液管家酶样蛋白(17%，25/147)、核糖体蛋白样蛋白(~31%，46/147)和杂类蛋白(~24%，35/147)。在其他类别中值得注意的包括抗菌肽(微藻毒素和蓖麻毒素)，以前在扁虱唾液中发现的肌球蛋白样蛋白，以及热休克扁虱唾液蛋白。这项研究中的数据为深入分析在大多数TBD病原体传播之前的第一个24小时内的肩袖吸食提供了基础。
Ixodes scapularis is arguably the most medically important tick species in the United States. This tick transmits 5 of the 14 human tick-borne disease (TBD) agents in the USA: Borrelia burgdorferi, Anaplasma phagocytophilum, B. miyamotoi, Babesia microti, and Powassan virus disease. Except for the Powassan virus disease, I. scapularis-vectored TBD agents require more than 24 h post attachment to be transmitted. This study describes identification of 24 h immunogenic I. scapularis tick saliva proteins, which could provide opportunities to develop strategies to stop tick feeding before transmission of the majority of pathogens. A 24 h fed female I. scapularis phage display cDNA expression library was biopanned using rabbit antibodies to 24 h fed I. scapularis female tick saliva proteins, subjected to next generation sequencing, de novo assembly, and bioinformatic analyses. A total of 182 contigs were assembled, of which ~19% (35/182) are novel and did not show identity to any known proteins in GenBank. The remaining ~81% (147/182) of contigs were provisionally identified based on matches in GenBank including ~18% (27/147) that matched protein sequences previously annotated as hypothetical and putative tick saliva proteins. Others include proteases and protease inhibitors (~3%, 5/147), transporters and/or ligand binding proteins (~6%, 9/147), immunogenic tick saliva housekeeping enzyme-like (17%, 25/147), ribosomal protein-like (~31%, 46/147), and those classified as miscellaneous (~24%, 35/147). Notable among the miscellaneous class include antimicrobial peptides (microplusin and ricinusin), myosin-like proteins that have been previously found in tick saliva, and heat shock tick saliva protein. Data in this study provides the foundation for in-depth analysis of I. scapularis feeding during the first 24 h, before the majority of TBD agents can be transmitted.
登录
查看更多内容
DOI：
10.1007/978-3-642-16861-1_2
发表时间：
2011-01-01
期刊：
EXTRACELLULAR MATRIX DEGRADATION
影响因子：
--
作者：
Broemme, Dieter;Wilson, Susan
通讯作者：
Wilson, Susan
DOI：
10.4269/ajtmh.2004.71.3.0700268
发表时间：
2004-09-01
影响因子：
3.3
作者：
Ebel, GD;Kramer, LD
通讯作者：
Kramer, LD
影响因子：
3.8
作者：
Abdulla, Maha-Hamadien;O'Brien, Theresa;McKerrow, James H.
通讯作者：
McKerrow, James H.
影响因子：
2.5
作者：
de la Fuente, Jose;Almazan, Consuelo;Willadsen, Peter
通讯作者：
Willadsen, Peter
影响因子：
158.5
作者：
BENACH, JL;BOSLER, EM;KASLOW, RA
通讯作者：
KASLOW, RA