Malignant and tuberculous pleural effusions: immunophenotypic cellular characterization.
Malignant and tuberculous pleural effusions: immunophenotypic cellular characterization.
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DOI：
10.1590/s1807-59322008000500012
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Saldiva PH
中科院分区：
文献类型：
--
作者：
Aguiar LM;Antonangelo L;Vargas FS;Zerbini MC;Sales MM;Uip DE;Saldiva PH
结核和癌症是胸腔积液的主要原因。胸膜受累与免疫细胞向胸膜腔的迁移有关。我们试图描述结核病或恶性肿瘤患者胸腔积液和外周血中白细胞的免疫表型。研究了30例结核病（14例）或恶性肿瘤（16例）患者。对照组为20例健康献血员。与PB淋巴细胞相比，恶性藻红蛋白胸腔积液显示出较高的CD 3、CD 4、CD 3CD 45 RO和CD 20 CD 25淋巴细胞百分比，而较低的CD 3CD 25和CD 20 HLA-DR百分比。与PB相比，结核性胸腔积液中共表达CD 3、CD 4、CD 3 CD 45 RO、CD 3 TCR αβ、CD 3 CD 28和CD 20的淋巴细胞百分比较高，而CD 14、CD 8和CD 3 TCR γδ阳性淋巴细胞百分比较低。恶性胸腔积液中CD 14的表达明显高于结核性胸腔积液中CD 3和CD 3CD 95 L的表达。结核病患者外周血细胞CD 14、CD 20、CD 25、CD 3、CD 95 L表达增高。与对照细胞相比，结核病和癌症患者外周血细胞中CD 3 CD 4和CD 3 CD 28阳性细胞的比例较低，而CD 3 CD 8、CD 3 CD 25和CD 3 CD 80阳性细胞的比例较高。恶性肿瘤患者外周血中富含具有辅助/诱导T细胞表型的淋巴细胞，其主要是记忆细胞。CD 14阳性细胞多见于恶性胸腔积液，而表达Fas配体的CD 3阳性细胞多见于结核性胸腔积液。
Tuberculosis and cancer are the main causes of pleural effusion. Pleural involvement is associated with migration of immune cells to the pleural cavity. We sought to characterize the immunophenotype of leukocytes in the pleural effusion and peripheral blood of patients with tuberculosis or malignancy. Thirty patients with tuberculosis (14) or malignancy (16) were studied. A control group included 20 healthy blood donors. Malignant phycoerythrin pleural effusions showed higher percentages of CD3, CD4, CD3CD45RO, and CD20CD25 lymphocytes and lower percentages of CD3CD25 and CD20HLA-DR when compared to PB lymphocytes. Compared to PB, tuberculous effusions had a higher percentage of lymphocytes that co-expressed CD3, CD4, CD3CD45RO, CD3TCRαβ, CD3CD28, and CD20 and a lower percentage of CD14, CD8 and CD3TCRγδ-positive lymphocytes. Malignant effusions presented higher expression of CD14 whereas tuberculous effusions had higher expression of CD3 and CD3CD95L. Peripheral blood cells from tuberculosis patients showed higher expression of CD14, CD20CD25 and CD3CD95L. Compared with the control cells, tuberculosis and cancer peripheral blood cells presented a lower percentage of CD3CD4 and CD3CD28-positive cells as well as a higher percentage of CD3CD8, CD3CD25 and CD3CD80-positive cells. Tuberculous and malignant peripheral blood is enriched with lymphocytes with a helper/inducer T cell phenotype, which are mainly of memory cells. CD14-positive cells were more frequently found in malignant effusions, while CD3-positive cells expressing Fas ligand were more frequently found in tuberculous effusions.
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影响因子：
4.4
作者：
Dlugovitzky, D;Rateni, L;Bottasso, O
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Bottasso, O
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作者：
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