Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea).
Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea).
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DOI：
10.1186/1471-2229-7-9
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发表时间：
2007-02-27
影响因子：
5.3
通讯作者：
Lopes CR
中科院分区：
文献类型：
--
作者：
Gimenes MA;Hoshino AA;Barbosa AV;Palmieri DA;Lopes CR
花生属包括花生（栽培花生）和用于花生育种或作为饲料的野生物种。分子标记已被用于该属的多项研究，但微卫星标记仅在少数调查中使用。微卫星信息量很大，对评估遗传变异、分析交配系统和遗传作图都很有用。本研究的目的是开发A. hypogaea微卫星位点，并评估这些标记的转移到其他花生物种。利用16份A. hypogaea。在A.使用微卫星标记的hypogaea高于其他标记。标记的交叉可转移性也很高。用引物Ah11对17个野生花生属种的DNA片段进行测序，结果表明，几乎所有野生花生属种都具有与A. hypogaea。序列分析表明，野生种与A. hypogaea和在其微卫星位点中发现的重复数。这些结果表明，来自A. hypogaea有多种用途。A.与其它标记如RFLP、RAPD和AFLP相比，使用微卫星标记可以检测hypogaea种质。利用微卫星引物对A. hypogaea表现出很高的转移率，以其他物种的属。这些引物对为评价花生属物种的遗传变异和交配系统提供了重要的工具。
The genus Arachis includes Arachis hypogaea (cultivated peanut) and wild species that are used in peanut breeding or as forage. Molecular markers have been employed in several studies of this genus, but microsatellite markers have only been used in few investigations. Microsatellites are very informative and are useful to assess genetic variability, analyze mating systems and in genetic mapping. The objectives of this study were to develop A. hypogaea microsatellite loci and to evaluate the transferability of these markers to other Arachis species. Thirteen loci were isolated and characterized using 16 accessions of A. hypogaea. The level of variation found in A. hypogaea using microsatellites was higher than with other markers. Cross-transferability of the markers was also high. Sequencing of the fragments amplified using the primer pair Ah11 from 17 wild Arachis species showed that almost all wild species had similar repeated sequence to the one observed in A. hypogaea. Sequence data suggested that there is no correlation between taxonomic relationship of a wild species to A. hypogaea and the number of repeats found in its microsatellite loci. These results show that microsatellite primer pairs from A. hypogaea have multiple uses. A higher level of variation among A. hypogaea accessions can be detected using microsatellite markers in comparison to other markers, such as RFLP, RAPD and AFLP. The microsatellite primers of A. hypogaea showed a very high rate of transferability to other species of the genus. These primer pairs provide important tools to evaluate the genetic variability and to assess the mating system in Arachis species.
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影响因子：
3.1
作者：
HALWARD, TM;STALKER, HT;KOCHERT, G
通讯作者：
KOCHERT, G
DOI：
10.1073/pnas.91.1.88
发表时间：
1994-01-04
影响因子：
11.1
作者：
KANDPAL, RP;KANDPAL, G;WEISSMAN, SM
通讯作者：
WEISSMAN, SM
影响因子：
1.9
作者：
He, GH;Prakash, CS
通讯作者：
Prakash, CS
影响因子：
1.9
作者：
BIANCHIHALL, CM;KEYS, RD;MURPHY, JP
通讯作者：
MURPHY, JP
影响因子：
2.1
作者：
Gimenes, Marcos A.;Lopes, Catalina R.;Valls, Jose F.M.
通讯作者：
Valls, Jose F.M.