Material stiffness effects on neurite alignment to photopolymerized micropatterns.
Material stiffness effects on neurite alignment to photopolymerized micropatterns.
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DOI：
10.1021/bm501019s
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发表时间：
2014-10-13
影响因子：
6.2
通讯作者：
Guymon, C. Allan
中科院分区：
文献类型：
--
作者：
Tuft, Bradley W.;Zhang, Lichun;Xu, Linjing;Hangartner, Austin;Leigh, Braden;Hansen, Marlan R.;Guymon, C. Allan
引导神经突起生长到神经假体刺激元件的近距离，如视网膜或人工耳蜗(CI)的能力，可能会提高设备的性能，并克服当前的空间信号分辨率障碍。在这项工作中，螺旋神经节神经元(SGN)是顺式刺激的靶神经元，培养在具有不同基质硬度的光聚合微图案上，以确定刚性对突起与物理线索对齐的影响。通过平行线间距光栅对预聚体配方进行光掩模，通过简单、快速的光聚合步骤在甲基丙烯酸酯薄膜表面产生微图案。评价了两个具有显著不同表面润湿性能的甲基丙烯酸酯系列，一个非极性HMA-co-HDDMA系列和一个极性PEGDMA-co-EGDMA系列。基于光掩模带间距，在每个甲基丙烯酸酯系列上保持等效的图案周期，并且使用UV固化方法提供的时间控制，将所有组合物的特征幅度调至2μm的深度。用扫描电子显微镜和白光干涉法对其表面形貌进行了表征。所有微图案膜从溶液中吸附相同数量的层粘连蛋白，当比较底物组成时，没有观察到SGN存活率的显著差异。两个甲基丙烯酸酯系列的SGN轴突取向均随材料模数的增加而显著增加。有趣的是，SGN神经突起对材料僵硬提示的反应(GPA)比通常归因于神经环境(KPA)的高一个数量级。能够理解神经突起对工程物理线索和力学性质(如基质硬度)的反应，将有助于开发先进的生物材料，指导新生突起生长，以解决当前神经假体的空间信号分辨率限制。
The ability to direct neurite growth into a close proximity of stimulating elements of a neural prosthesis, such as a retinal or cochlear implant (CI), may enhance device performance and overcome current spatial signal resolution barriers. In this work, spiral ganglion neurons (SGNs), which are the target neurons to be stimulated by CIs, were cultured on photopolymerized micropatterns with varied matrix stiffnesses to determine the effect of rigidity on neurite alignment to physical cues. Micropatterns were generated on methacrylate thin film surfaces in a simple, rapid photopolymerization step by photomasking the prepolymer formulation with parallel line–space gratings. Two methacrylate series, a nonpolar HMA-co-HDDMA series and a polar PEGDMA-co-EGDMA series, with significantly different surface wetting properties were evaluated. Equivalent pattern periodicity was maintained across each methacrylate series based on photomask band spacing, and the feature amplitude was tuned to a depth of 2 μm amplitude for all compositions using the temporal control afforded by the UV curing methodology. The surface morphology was characterized by scanning electron microscopy and white light interferometry. All micropatterned films adsorb similar amounts of laminin from solution, and no significant difference in SGN survival was observed when the substrate compositions were compared. SGN neurite alignment significantly increases with increasing material modulus for both methacrylate series. Interestingly, SGN neurites respond to material stiffness cues that are orders of magnitude higher (GPa) than what is typically ascribed to neural environments (kPa). The ability to understand neurite response to engineered physical cues and mechanical properties such as matrix stiffness will allow the development of advanced biomaterials that direct de novo neurite growth to address the spatial signal resolution limitations of current neural prosthetics.
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影响因子：
2.8
作者：
Clarke, Joseph C.;Tuft, Bradley W.;Clinger, John D.;Levine, Rachel;Figueroa, Lucas Sievens;Guymon, C. Allan;Hansen, Marlan R.
通讯作者：
Hansen, Marlan R.
影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M
影响因子：
3.4
作者：
Chou, Szu-Yuan;Cheng, Chao-Min;LeDuc, Philip R.
通讯作者：
LeDuc, Philip R.
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE