In vivo intravascular ultrasound-guided photoacoustic imaging of lipid in plaques using an animal model of atherosclerosis.
In vivo intravascular ultrasound-guided photoacoustic imaging of lipid in plaques using an animal model of atherosclerosis.
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DOI：
10.1016/j.ultrasmedbio.2012.08.006
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发表时间：
2012-12
影响因子：
2.9
通讯作者：
Emelianov, Stanislav
中科院分区：
文献类型：
--
作者：
Wang, Bo;Karpiouk, Andrei;Yeager, Doug;Amirian, James;Litovsky, Silvio;Smalling, Richard;Emelianov, Stanislav
我们提出了一项初步研究，证明超声引导血管内光声成像（IVPA）能够可视化体内动脉粥样硬化斑块中脂质沉积的深度分辨分布。基于近红外波长范围内脂质的光学吸收特性，采用单波长1720 nm的IVPA成像技术，对动脉粥样硬化动脉中的脂质含量进行了空间分辨的直接测量。通过将IVPA图像与空间共配血管内超声（IVUS）图像叠加，IVPA/IVUS联合图像用于可视化血管壁内脂质分布。超声引导下对渡边遗传性高脂血症（WHHL）兔腹主动脉进行了活体IVPA成像。随后，切除的兔主动脉充满红细胞（RBC）溶液，然后进行离体成像，并在成像横切面相邻的切片上获得组织学。为了证明IVPA/IVUS成像在未来临床应用的潜力，我们还对一个患病的人右冠状动脉（RCA）样本进行了成像。体内和离体IVPA图像均清晰显示动脉粥样硬化血管中的脂质分布。体内IVPA成像能够在WHHL兔动脉粥样硬化模型中识别弥漫性、富含脂质的斑块。此外，单波长IVPA成像能够在体外识别人RCA内的脂质核心。我们的研究结果表明，超声引导下的IVPA成像可以识别体内动脉粥样硬化斑块中的脂质。重要的是，IVPA/IVUS图像是在腔内血液存在的情况下获得的，不需要盐水冲洗或球囊阻塞。总之，我们的研究表明，超声引导下的IVPA成像可以潜在地用于血管壁和管腔解剖背景下的脂质沉积的深度分辨率可视化。因此，IVUS/ IVPA成像可能成为检测易破裂斑块的重要工具。
We present a preliminary study demonstrating the capability of ultrasound-guided intravascular photoacoustic (IVPA) imaging to visualize the depth-resolved distribution of lipid deposits in atherosclerotic plaques in vivo. Based on the characteristic optical absorption of lipid in the near infrared wavelength range, IVPA imaging at a single, 1720 nm, wavelength was used to provide a spatially resolved direct measurement of lipid content in atherosclerotic arteries. By overlaying an IVPA image with a spatially co-registered intravascular ultrasound (IVUS) image, the combined IVPA/IVUS image was used to visualize the distribution of lipid within the vessel wall. Ultrasound-guided IVPA imaging was performed in vivo in the abdominal aorta of a Watanabe heritable hyperlipidemic (WHHL) rabbit. Subsequently, the excised rabbit aorta filled with a solution of red blood cells (RBC) was then imaged ex vivo, and the histology was obtained in the section adjacent to the imaged cross-section. To demonstrate the potential of future clinical application of IVPA/IVUS imaging, a sample of diseased human right coronary artery (RCA) was also imaged. Both in vivo and ex vivo IVPA images clearly showed the distribution of lipid in the atherosclerotic vessels. In vivo IVPA imaging was able to identify diffuse, lipid-rich plaques in the WHHL rabbit model of atherosclerosis. Furthermore, IVPA imaging at a single wavelength was able to identify the lipid core within the human RCA ex vivo. Our results demonstrate that ultrasound-guided IVPA imaging can identify lipid in atherosclerotic plaques in vivo. Importantly, the IVPA/IVUS images were obtained in presence of luminal blood and no saline flush or balloon occlusion was required. Overall, our studies suggest that ultrasound-guided IVPA imaging can potentially be used for depth-resolved visualization of lipid deposits within the anatomical context of the vessel wall and lumen. Therefore, IVUS/ IVPA imaging may become an important tool for the detection of rupture-prone plaques.
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DOI：
10.1016/s0735-1097(02)01767-9
发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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