Co-culture with Schwann cells is an effective way for adipose-derived stem cells neural transdifferentiation.
Co-culture with Schwann cells is an effective way for adipose-derived stem cells neural transdifferentiation.
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与雪旺细胞共培养是脂肪干细胞神经转分化的有效途径。
DOI：
10.5114/aoms.2010.13885
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发表时间：
2010-04-30
期刊：
影响因子：
--
通讯作者：
Yuan Q
中科院分区：
文献类型：
--
作者：
Liao D;Gong P;Li X;Tan Z;Yuan Q
脂肪源性干细胞（ADSCs）在体外可在某些生长因子的作用下完成神经转分化。骨髓基质干细胞（BMSCs）只有与雪旺细胞（Schwann cells，SC）共同培养才能实现向神经元的转分化，我们前期的研究也证实了脂肪干细胞（ADSCs）也可以实现向神经元的转分化。本文旨在研究共培养的神经诱导效率是否与使用化学物质或化学物质与某些生长因子组合的其他策略一样高。我们分离并扩增了成年SD大鼠和1 ~ 2日龄SD大鼠坐骨神经的脂肪干细胞，分别用2%二甲基亚砜（DMSO）和DMSO联合生长因子诱导脂肪干细胞向神经元转分化。同时，我们在Transwell培养皿中共培养ADSCs和SCs，不进行细胞间接触。采用免疫组化和RT-PCR方法观察ADSCs的神经转分化情况。采用实时荧光定量PCR方法比较三种方案中S100、nestin和GFAP基因的表达差异。免疫组化和RT-PCR结果均证明，上述三种方法均可诱导脂肪干细胞向神经元转分化。实时荧光定量PCR进一步显示，共培养与DMSO联合生长因子诱导的上述三个基因的基因表达相对量无统计学差异（p > 0.05），但均与单独DMSO诱导的基因表达相对量有统计学差异（p < 0.05）。脂肪干细胞与干细胞共培养可能是一种简单、有效、实用的脂肪干细胞向神经元转分化的方法。
Adipose-derived stem cells (ADSCs) could accomplish neural transdifferentiation with the presence of certain growth factors in vitro. It has been proved that bone marrow stromal cells (BMSCs) can realize neural transdifferentiation only by being co-cultured with Schwann cells (SCs), and in our former studies we have confirmed that ADSCs could do so too. This paper aims to investigate whether the neural induction efficiency of co-culture is as high as that of other strategies using chemicals or chemicals combined with some growth factors. We isolated and multiplied ADSCs from adult Sprague-Dawley rats, and SCs from sciatic nerves of 1-to-2-day-old Sprague-Dawley rat pups, then induced ADSCs neural transdifferentiation through 2% dimethyl sulphoxide (DMSO) and DMSO combined with growth factors. Meanwhile we co-cultured ADSCs and SCs in Transwell culture dishes without intercellular contacts. Immunostaining and RT-PCR were adopted to investigate the neural transdifferentiation of ADSCs. Then we compared the expression differences for genes S100, nestin and GFAP of the above three protocols by real-time PCR. Both immunostaining and RT-PCR proved that ADSCs could accomplish neural transdifferentiation through each of the above three protocols. And real-time PCR further shows that the gene expression relative quantities for the above three genes are not statistically different between co-culture and induction through DMSO combined with growth factors (p > 0.05), but both of them are statistically different from induction only by DMSO (p < 0.05). Co-culturing ADSCs and SCs may be a simple, effective and practical way for ADSCs neural transdifferentiation.
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影响因子：
64.8
作者：
Wang, X;Willenbring, H;Grompe, M
通讯作者：
Grompe, M
影响因子：
64.8
作者：
Alvarez-Dolado, M;Pardal, R;Alvarez-Buylla, A
通讯作者：
Alvarez-Buylla, A
影响因子：
2.9
作者：
Ning, Hongxiu;Lin, Guiting;Lin, Ching-Shwun
通讯作者：
Lin, Ching-Shwun
影响因子：
0.8
作者：
Li, Xiaojie;Liao, Dapeng;Tan, Zhen
通讯作者：
Tan, Zhen
DOI：
10.1016/s0006-291x(02)00469-2
发表时间：
2002-06-07
影响因子：
3.1
作者：
Safford, KM;Hicok, KC;Rice, HE
通讯作者：
Rice, HE