Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile.
Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile.
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DOI：
10.1080/19490976.2021.1973835
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发表时间：
2021-01
期刊：
影响因子：
12.2
通讯作者：
Kurihara S
中科院分区：
文献类型：
--
作者：
Hirano R;Sakanaka M;Yoshimi K;Sugimoto N;Eguchi S;Yamauchi Y;Nara M;Maeda S;Ami Y;Gotoh A;Katayama T;Iida N;Kato T;Ohno H;Fukiya S;Yokota A;Nishimoto M;Kitaoka M;Nakai H;Kurihara S
某些现有的益生元旨在促进肠道中有益细菌的生长，也促进其他主要细菌的生长。因此，分析了β-半乳糖苷对肠道细菌的促生长作用。半乳糖基-β 1，4-L-鼠李糖（Gal-β 1，4-Rha）选择性地促进双歧杆菌的生长。长双歧杆菌亚种longum 105-A（JCM 31944）具有属于糖的ATP结合盒转运蛋白的多种溶质结合蛋白。文库中的每个菌株11个B。长双歧杆菌婴儿亚种在含有Gal-β 1，4-Rha作为唯一碳源的培养基中培养其中溶质结合蛋白的每个基因均被破坏的Longum突变体，并且与野生型菌株相比，只有BL105A_0502基因破坏突变体显示出延迟和降低的生长。BL105A_0502同源物在双歧杆菌中高度保守。在含Gal-β 1，4-Rha的培养基中，长双歧杆菌（Bifidobacterium longum subsp. BL105A_0502的同源蛋白BLIJ_2090能抑制艰难梭菌的生长，而BLIJ_2090基因缺失的突变株则不能。在体内，施用B。Gal-β 1，4-Rha可减轻C.艰难梭菌感染相关的小鼠体重减轻。我们已经成功地筛选出Gal-β 1，4-Rha作为下一代益生元候选物，它可以特异性地促进有益细菌的生长，而不会促进主要细菌和病原体的生长。
Certain existing prebiotics meant to facilitate the growth of beneficial bacteria in the intestine also promote the growth of other prominent bacteria. Therefore, the growth-promoting effects of β-galactosides on intestinal bacteria were analyzed. Galactosyl-β1,4-l-rhamnose (Gal-β1,4-Rha) selectively promoted the growth of Bifidobacterium. Bifidobacterium longum subsp. longum 105-A (JCM 31944) has multiple solute-binding proteins belonging to ATP-binding cassette transporters for sugars. Each strain in the library of 11 B. longum subsp. longum mutants, in which each gene of the solute-binding protein was disrupted, was cultured in a medium containing Gal-β1,4-Rha as the sole carbon source, and only the BL105A_0502 gene-disruption mutant showed delayed and reduced growth compared to the wild-type strain. BL105A_0502 homolog is highly conserved in bifidobacteria. In a Gal-β1,4-Rha-containing medium, Bifidobacterium longum subsp. infantis JCM 1222T, which possesses BLIJ_2090, a homologous protein to BL105A_0502, suppressed the growth of enteric pathogen Clostridioides difficile, whereas the BLIJ_2090 gene-disrupted mutant did not. In vivo, administration of B. infantis and Gal-β1,4-Rha alleviated C. difficile infection-related weight loss in mice. We have successfully screened Gal-β1,4-Rha as a next-generation prebiotic candidate that specifically promotes the growth of beneficial bacteria without promoting the growth of prominent bacteria and pathogens.
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DOI：
10.1128/genomea.01311-14
发表时间：
2014-12-18
期刊：
Genome announcements
影响因子：
--
作者：
Kanesaki Y;Masutani H;Sakanaka M;Shiwa Y;Fujisawa T;Nakamura Y;Yokota A;Fukiya S;Suzuki T;Yoshikawa H
通讯作者：
Yoshikawa H
影响因子：
6.4
作者：
Bottacini F;Ventura M;van Sinderen D;O'Connell Motherway M
通讯作者：
O'Connell Motherway M
影响因子：
5.4
作者：
CROCIANI, F;ALLESANDRINI, A;BIAVATI, B
通讯作者：
BIAVATI, B
影响因子：
64.8
作者：
Fukuda, Shinji;Toh, Hidehiro;Ohno, Hiroshi
通讯作者：
Ohno, Hiroshi
影响因子：
11.8
作者：
McDonald, L. Clifford;Gerding, Dale N.;Wilcox, Mark H.
通讯作者：
Wilcox, Mark H.