Technique for internal channelling of hydroentangled nonwoven scaffolds to enhance cell penetration.
Technique for internal channelling of hydroentangled nonwoven scaffolds to enhance cell penetration.
复制标题
用于内部通道非织造支架的内部通道技术，以增强细胞渗透。
DOI：
10.1177/0885328212445077
复制
发表时间：
2013-08
影响因子：
2.9
通讯作者：
Russell SJ
中科院分区：
文献类型：
--
作者：
Durham ER;Ingham E;Russell SJ
厚的、高孔隙率的支架的一个重要要求是在体外培养过程中最大限度地使细胞渗透到内部并避免坏死。迄今为止，无纺布支架孔结构的可重复性控制已被证明是具有挑战性的。报道了一种新的通道支架制造工艺，该工艺基于纤维在丝状模板周围的水射流缠结（水缠结），形成随后被移除的连贯支架。纵向导向的通道使用直径220微米的圆柱形模板在支架内进行控制。在本案例研究中，制备了由聚乳酸组成的通道支架，并在体外对其进行了评价。环境扫描电镜和微CT （x射线微断层扫描）证实，在人类真皮成纤维细胞培养14周前后，支架横截面上出现了通道开口。第11周的组织学显示，这些通道促进了细胞在支架内部的渗透和分布。在第14周，细胞基质在内部通道壁上明显沉积，入口仍未被细胞基质堵塞，这表明可以维持向支架内部传递营养物质的扩散管道。
An important requirement in thick, high-porosity scaffolds is to maximise cellular penetration into the interior and avoid necrosis during culture in vitro. Hitherto, reproducible control of the pore structure in nonwoven scaffolds has proved challenging. A new, channelled scaffold manufacturing process is reported based on water jet entanglement of fibres (hydroentangling) around filamentous template to form a coherent scaffold that is subsequently removed. Longitudinally-oriented channels were introduced within the scaffold in controlled proximity using 220 µm diameter cylindrical templates. In this case study, channelled scaffolds composed of poly(l-lactic acid) were manufactured and evaluated in vitro. Environmental scanning electron microscope and µCT (X-ray microtomography) confirmed channel openings in the scaffold cross-section before and after cell culture with human dermal fibroblasts up to 14 weeks. Histology at week 11 indicated that the channels promoted cell penetration and distribution within the scaffold interior. At week 14, cellular matrix deposition was evident in the internal channel walls and the entrances remained unoccluded by cellular matrix suggesting that diffusion conduits for mass transfer of nutrient to the scaffold interior could be maintained.
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影响因子：
4.9
作者：
Wang, Junping;Valmikinathan, Chandra M.;Yu, Xiaojun
通讯作者：
Yu, Xiaojun
影响因子：
6.2
作者：
Nazhat, Showan N.;Abou Neel, Ensanya A.;Brown, Robert A.
通讯作者：
Brown, Robert A.
影响因子：
--
作者：
Sachlos, Eleftherios;Gotora, Duce;Czernuszka, Jan T.
通讯作者：
Czernuszka, Jan T.
DOI：
10.1115/1.4002429
发表时间：
2010-10-01
影响因子：
1.7
作者：
Ahn, Geunseon;Park, Jeong Hun;Cho, Dong-Woo
通讯作者：
Cho, Dong-Woo
影响因子：
3.7
作者：
Freed, LE;Hollander, AP;Vunjak-Novakovic, G
通讯作者：
Vunjak-Novakovic, G