The first identified cathelicidin from tree frogs possesses anti-inflammatory and partial LPS neutralization activities.
The first identified cathelicidin from tree frogs possesses anti-inflammatory and partial LPS neutralization activities.
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首次从树蛙中鉴定出具有抗炎和部分 LPS 中和活性的导管素
DOI：
10.1007/s00726-017-2449-7
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发表时间：
2017-09
期刊：
影响因子：
3.5
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Mu L;Zhou L;Yang J;Zhuang L;Tang J;Liu T;Wu J;Yang H
截至2017年2月，在AmphibiaWeb数据库中已经描述了大约7639种两栖动物。然而，迄今为止，仅从10种两栖动物中鉴定出20种类抗菌肽。这些肽中有一半是从基因组序列中鉴定出来的，尚未被功能表征。本研究从葛树蛙的皮肤中纯化了一种新型的cathelicidin-PP肽。Cathelicidin-PP是序列ASENGKCNLLCLVKKKLRAVGNVIKTVVGKIA的32位残基肽。圆二色性光谱分析表明，抗菌肽- pp在模拟膜溶液中主要采用β-片结构。抗菌肽- pp对细菌和真菌，尤其是革兰氏阴性菌具有很强的抗菌活性。同时对哺乳动物细胞具有较低的细胞毒性。扫描电镜分析表明，抗菌肽- pp通过破坏细菌细胞膜完整性杀死细菌。此外，cathelicidin-PP通过抑制脂多糖（LPS）介导的一氧化氮和促炎细胞因子、肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6的产生，具有显著的抗炎功能。MAPKs （ERK、JNK和p38）和NF-κB信号通路参与抗炎作用。Cathelicidin-PP以剂量依赖的方式引起LPS的部分中和。定量PCR结果显示，树蛙感染细菌后，免疫相关组织中cathelicidin-PP表达增加。综上所述，cathelicidin-PP是第一个从树蛙中鉴定出的cathelicidin样肽。我们的研究结果表明，除了直接杀菌能力外，cathelicidin-PP还具有免疫调节特性，包括部分中和LPS，抑制炎症细胞因子的产生。
As of February 2017, approximately 7639 amphibian species have been described in the AmphibiaWeb database. However, only 20 cathelicidin-like antimicrobial peptides have been identified to date from 10 amphibian species. Half of these peptides were identified from genome sequences and have not yet been functionally characterized. In this study, a novel cathelicidin-like peptide designated cathelicidin-PP was purified from the skin of tree frogPolypedates puerensis. Cathelicidin-PP is a 32 residue peptide of sequence ASENGKCNLLCLVKKKLRAVGNVIKTVVGKIA. Circular dichroism spectroscopy indicated that cathelicidin-PP mainly adopts a β-sheet structure in membrane-mimetic solutions. Cathelicidin-PP exhibits potent antimicrobial activity against bacteria and fungi, especially Gram-negative bacteria. Meanwhile, it shows low cytotoxicity toward mammalian cells. Scanning electron microscopy analysis indicated that cathelicidin-PP kills bacteria through the disruption of the bacterial cell membrane integrity. Furthermore, cathelicidin-PP exerts significant anti-inflammatory functions by inhibiting the lipopolysaccharide (LPS)-mediated generation of nitric oxide and pro-inflammatory cytokines, tumor necrosis factor-α, interleukin-1β, and interleukin-6. The MAPKs (ERK, JNK, and p38) and NF-κB signaling pathways are involved in the anti-inflammatory effect. Cathelicidin-PP caused partial neutralization of LPS in a dose-dependent manner. Quantitative PCR indicated that infection of tree frogs with bacteria causes increased expression of cathelicidin-PP in immune-related tissues. Taken together, cathelicidin-PP is the first identified cathelicidin-like peptide from tree frogs. Our findings demonstrate that in addition to direct bactericidal capacity, cathelicidin-PP also possesses immunomodulatory properties, including partial neutralization of LPS, and inhibiting the production of inflammatory cytokines.
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来自牛蛙 Rana catesbeiana 的 Cathelicidins 为肽抗生素设计提供了新的模板
影响因子：
3.7
作者：
Ling G;Gao J;Zhang S;Xie Z;Wei L;Yu H;Wang Y
通讯作者：
Wang Y
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
DOI：
10.1046/j.1432-1033.2003.03584.x
发表时间：
2003-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Vanhoye, D;Bruston, F;Amiche, M
通讯作者：
Amiche, M
影响因子：
2.5
作者：
Wallace, BA
通讯作者：
Wallace, BA
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD