Mutations in a beta-tubulin disrupt spindle orientation and microtubule dynamics in the early Caenorhabditis elegans embryo.
Mutations in a beta-tubulin disrupt spindle orientation and microtubule dynamics in the early Caenorhabditis elegans embryo.
复制标题
β-微管蛋白的突变破坏了早期秀丽隐杆线虫胚胎中的纺锤体方向和微管动力学。
DOI：
--
复制
发表时间：
2003
影响因子：
3.3
通讯作者：
C. Hunter
中科院分区：
文献类型：
--
作者：
Amanda Wright;C. Hunter
早期秀丽隐杆线虫胚胎含有丰富的转录本的两个α-和两个β-微管蛋白，提出了一个问题，是否每个亚型执行专门的功能或只是有助于总微管蛋白水平。我们发现了两个隐性的β-微管蛋白的互补等位基因，它们在早期胚胎中破坏了核中心体的向心和旋转，这最初表明这种微管蛋白tbb-2具有特殊的功能。然而，胚胎从tbb-2缺失蠕虫不具有核中心体集中和旋转的缺陷，这表明互补等位基因不是无效突变。两个互补等位基因对微管动力学有不同的影响，并显示与两种胚胎表达的α-微管蛋白的等位基因特异性相互作用：其中一个等位基因导致微管冷稳定并对微管解聚药物苯菌灵具有抗性，而另一个等位基因导致微管聚合中的细胞周期特异性缺陷。针对所有四个胚胎表达的微管蛋白基因的基因特异性RNA干扰单独和所有双组合显示，在早期胚胎中的微管蛋白亚型在很大程度上是功能冗余的，除了tbb-2。在第一次细胞分裂后期，中心体稳定需要TBB-2，这表明TBB-2可能专门与细胞皮层相互作用。
The early Caenorhabditis elegans embryo contains abundant transcripts for two alpha- and two beta-tubulins, raising the question of whether each isoform performs specialized functions or simply contributes to total tubulin levels. Our identification of two recessive, complementing alleles of a beta-tubulin that disrupt nuclear-centrosome centration and rotation in the early embryo originally suggested that this tubulin, tbb-2, has specialized functions. However, embryos from tbb-2 deletion worms do not have defects in nuclear-centrosome centration and rotation suggesting that the complementing alleles are not null mutations. Both complementing alleles have distinct effects on microtubule dynamics and show allele-specific interactions with the two embryonically expressed alpha-tubulins: One of the alleles causes microtubules to be cold stable and resistant to the microtubule-depolymerizing drug benomyl, whereas the other causes cell cycle-specific defects in microtubule polymerization. Gene-specific RNA interference targeting all four embryonically expressed tubulin genes singly and in all double combinations showed that the tubulin isoforms in the early embryo are largely functionally redundant with the exception of tbb-2. tbb-2 is required for centrosome stabilization during anaphase of the first cell division, suggesting that tbb-2 may be specialized for interactions with the cell cortex.
登录
查看更多内容
影响因子：
4
作者：
S. C. Schuyler;David Pellman
通讯作者：
S. C. Schuyler;David Pellman
影响因子：
3.3
作者：
Richards, KL;Anders, KR;Botstein, D
通讯作者：
Botstein, D
影响因子：
10.5
作者：
SAVAGE, C;HAMELIN, M;CHALFIE, M
通讯作者：
CHALFIE, M
影响早期秀丽隐杆线虫胚胎减数分裂和有丝分裂的突变。
影响因子：
3.3
作者：
Mains,PE;Kemphues,KJ;Sprunger,SA;Sulston,IA;Wood,WB
通讯作者：
Wood,WB