Controlled release and targeting of polypeptide-deposited liposomes by enzymatic degradation
Controlled release and targeting of polypeptide-deposited liposomes by enzymatic degradation
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通过酶降解控制多肽沉积脂质体的释放和靶向
DOI：
10.1038/s41428-019-0232-1
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发表时间：
2019
期刊：
影响因子：
2.8
通讯作者：
Fujimoto Keiji
中科院分区：
文献类型：
--
作者：
Fukui Yuuka;Otsuka Hikari;Fujimoto Keiji
我们制备了具有酶降解性的生物基纳米胶囊，其通过酶和多肽在脂质体表面的逐层沉积而产生。在这里，我们展示了两种不同的系统的基础上的酶降解的聚合物层。首先，在带负电荷的脂质体上进行胰蛋白酶和可被胰蛋白酶裂解的聚精氨酸（PArg）的沉积。PA的酶促裂解导致脂质膜暴露，这促进了货物的释放。接下来，我们尝试通过酶促降解来降解多层胶囊壁的外聚合物层以显示内聚合物层。这种方法能够通过显示的聚合物层和目标羟基磷灰石（HAp）之间的亲和力积累和靶向纳米胶囊。聚合物壁由聚-L-谷氨酸（PGlu）组成的内层和由胰蛋白酶和PArg组成的外层构建到脂质体上。胰蛋白酶降解外膜PArg后，膜表面呈现出内膜PGlu，具有骨靶向性。此外，聚合物壁由PArg内层和胃蛋白酶和PGlu外层构成。胃蛋白酶降解外层的PGl，使内层的PGr在表面达到穿透细胞的活性。
We prepared biobased nanocapsules with enzymatic degradability, which were generated by the layer-by-layer deposition of enzymes and polypeptide over the liposomal surface. Here, we demonstrate two different systems based on the enzymatic degradation of polymer layers. First, the deposition of trypsin and polyarginine (PArg), which is cleavable by trypsin, was carried out over a negatively charged liposome. The enzymatic cleavage of PArgresulted in exposure of the lipid membrane, which facilitated release of the cargo. Next, we attempted to degrade the outer polymer layer of the multilayered capsule wall to display the inner polymer layer by enzymatic degradation. This approach enabled the accumulation and targeting of the nanocapsules through the affinity between the displayed polymer layer and the target hydroxyapatite (HAp). The polymer wall was constructed with an inner layer consisting of poly-L-glutamic acid (PGlu) and an outer layer consisting of trypsin and PArgonto the liposome. The degradation of the outer PArgby trypsin allowed the surface to display the inner PGlu, which has bone-targeting ability. In addition, the polymer wall was constructed from an inner layer of PArgand an outer layer of pepsin and PGlu. The degradation of the outer PGluby pepsin led to inner PArgon the surface to achieve cell-penetrating activity.
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影响因子：
5.5
作者：
Fujimoto, Keiji;Toyoda, Tomonori;Fukui, Yuuka
通讯作者：
Fukui, Yuuka
影响因子：
3.9
作者：
Fukui, Yuuka;Fujimoto, Keiji
通讯作者：
Fujimoto, Keiji
影响因子：
--
作者：
Fukui, Yuuka
通讯作者：
Fukui, Yuuka
使用全身方法根据散射数据计算生物聚合物的流体动力学参数
DOI：
10.1007/bfb0118014
发表时间：
1997
期刊：
The Biochemical journal
影响因子：
--
作者：
H. Durchschlag;P. Zipper
通讯作者：
P. Zipper
光诱导双层囊泡的不稳定
影响因子：
15
作者：
D. A. Frankel;H. Lamparski;U. Liman;D. O'BRIEN
通讯作者：
D. O'BRIEN