Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate.
Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate.
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DOI：
10.1186/1754-1611-8-14
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发表时间：
2014
影响因子：
5.6
通讯作者：
Cernea V
中科院分区：
文献类型：
--
作者：
Brie IC;Soritau O;Dirzu N;Berce C;Vulpoi A;Popa C;Todea M;Simon S;Perde-Schrepler M;Virag P;Barbos O;Chereches G;Berce P;Cernea V
开发能够控制细胞活性并指导其命运的新型生物材料是工程化功能性骨组织的必要条件。添加生物活性材料可以促进新骨形成和更好的骨整合。在这项比较研究中，对三种类型的钛（Ti）植入物进行了体外生物相容性试验：总孔隙率为25%的Ti6 Al 7 Nb植入物作为对照，使用羟基磷灰石（Ti HA）和硅钛酸盐（Ti SiO2）的溶胶-凝胶法渗透植入物。观察人成骨细胞的粘附、生长和分化行为。这两种涂层方法提供了不同的形态和化学性质（SEM和EDX分析）。与Ti SiO2和多孔无涂层Ti植入物相比，Ti HA支架上第一个小时的细胞附着较慢。Alcohol blue试验和总蛋白质含量评估发现，在第8-9天出现代谢活性峰值，Ti SiO2植入物具有优势。通过骨桥蛋白（OP）表达（ELISA和免疫细胞化学）、碱性磷酸酶（ALP）活性、钙沉积（茜素红）、胶原合成（SIRCOL试验和免疫细胞化学染色）和骨钙素（OC）表达评价的成骨细胞分化和新生矿化，突出了Ti HA植入物的更高骨传导能力。在对照Ti和Ti SiO2植入物上，发现在简单成骨分化培养基中培养的细胞的可溶性胶原水平较高。骨钙素（OC），终末成骨细胞分化的标志物，最强烈地表达在钛硅种植体上培养的成骨细胞。成骨细胞的行为取决于植入物的类型和培养条件。Ti SiO2支架通过增加胶原和非胶原蛋白（OP和OC）的产生维持成骨细胞粘附并促进分化。Ti HA植入物诱导细胞粘附和增殖的能力较低，但诱导早期矿化的能力增加。在分化培养基中添加生长因子BMP-2和TGFβ1并不能促进矿化过程。这两种类型的浸润都有其优点和局限性，可以根据需要修复的骨病变的局部情况加以利用。这些限制也可以通过与骨生成中涉及的生物分子的功能化方法来抵消。
The development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional bone tissues. Adding bioactive materials can improve new bone formation and better osseointegration. Three types of titanium (Ti) implants were tested for in vitro biocompatibility in this comparative study: Ti6Al7Nb implants with 25% total porosity used as controls, implants infiltrated using a sol–gel method with hydroxyapatite (Ti HA) and silicatitanate (Ti SiO2). The behavior of human osteoblasts was observed in terms of adhesion, cell growth and differentiation. The two coating methods have provided different morphological and chemical properties (SEM and EDX analysis). Cell attachment in the first hour was slower on the Ti HA scaffolds when compared to Ti SiO2 and porous uncoated Ti implants. The Alamar blue test and the assessment of total protein content uncovered a peak of metabolic activity at day 8–9 with an advantage for Ti SiO2 implants. Osteoblast differentiation and de novo mineralization, evaluated by osteopontin (OP) expression (ELISA and immnocytochemistry), alkaline phosphatase (ALP) activity, calcium deposition (alizarin red), collagen synthesis (SIRCOL test and immnocytochemical staining) and osteocalcin (OC) expression, highlighted the higher osteoconductive ability of Ti HA implants. Higher soluble collagen levels were found for cells cultured in simple osteogenic differentiation medium on control Ti and Ti SiO2 implants. Osteocalcin (OC), a marker of terminal osteoblastic differentiation, was most strongly expressed in osteoblasts cultivated on Ti SiO2 implants. The behavior of osteoblasts depends on the type of implant and culture conditions. Ti SiO2 scaffolds sustain osteoblast adhesion and promote differentiation with increased collagen and non-collagenic proteins (OP and OC) production. Ti HA implants have a lower ability to induce cell adhesion and proliferation but an increased capacity to induce early mineralization. Addition of growth factors BMP-2 and TGFβ1 in differentiation medium did not improve the mineralization process. Both types of infiltrates have their advantages and limitations, which can be exploited depending on local conditions of bone lesions that have to be repaired. These limitations can also be offset through methods of functionalization with biomolecules involved in osteogenesis.
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影响因子：
3.8
作者：
Hannora, Ahmed E.;Mukasyan, Alexander S.;Mansurov, Zulkhair A.
通讯作者：
Mansurov, Zulkhair A.
影响因子：
4.2
作者：
Gericke, A;Qin, C;Boskey, AL
通讯作者：
Boskey, AL
影响因子：
4.9
作者：
Bosetti, M;Zanardi, L;Cannas, M
通讯作者：
Cannas, M
影响因子：
3.2
作者：
Geys, Jorina;Nemery, Benoit;Hoet, Peter H. M.
通讯作者：
Hoet, Peter H. M.
影响因子：
9.7
作者：
Hunter, Larry W.;Shiekh, Farooq A.;Lieske, John C.
通讯作者：
Lieske, John C.