Ecological influence of sediment bypass tunnels on macroinvertebrates in dam-fragmented rivers by DNA metabarcoding.
Ecological influence of sediment bypass tunnels on macroinvertebrates in dam-fragmented rivers by DNA metabarcoding.
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DOI：
10.1038/s41598-018-28624-2
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发表时间：
2018-07-05
影响因子：
4.6
通讯作者：
Watanabe K
中科院分区：
文献类型：
--
作者：
Serrana JM;Yaegashi S;Kondoh S;Li B;Robinson CT;Watanabe K
泥沙分流隧洞是一种导流结构，在水库运行过程中，通过将泥沙输送到下游河段，减少高流量时水库中的泥沙淤积。以往的研究监测下游河段的SBT业务的生态影响，建议SBT对河床沉积物条件和大型无脊椎动物群落的基础上，传统的形态调查的积极影响。基于形态学的大型无脊椎动物评估是昂贵和耗时的，大量的形态学上的神秘，小型和未描述的物种通常导致粗糙的分类鉴定。在这里，我们使用DNA元条形码分析来评估SBT操作对SBT出口下游大型无脊椎动物的影响，方法是通过估计具有操作SBT的水坝碎片化河流上游和下游位置之间的物种多样性和成对群落差异，与水坝碎片化（即，没有SBT）和自由流动的河流（即，无坝）。我们发现，大型底栖动物群落的差异性降低，增加操作时间和频率的SBT。SBT操作的这些因素影响河床特征的变化，例如沉积物关系，随后影响下游大型无脊椎动物群落恢复到各自的上游群落。大型无脊椎动物丰度使用形态学鉴定的标本是正相关的阅读丰度使用元条形码。这支持并加强了使用元条码数据的多样性分析的定量估计。
Sediment bypass tunnels (SBTs) are guiding structures used to reduce sediment accumulation in reservoirs during high flows by transporting sediments to downstream reaches during operation. Previous studies monitoring the ecological effects of SBT operations on downstream reaches suggest a positive influence of SBTs on riverbed sediment conditions and macroinvertebrate communities based on traditional morphology-based surveys. Morphology-based macroinvertebrate assessments are costly and time-consuming, and the large number of morphologically cryptic, small-sized and undescribed species usually results in coarse taxonomic identification. Here, we used DNA metabarcoding analysis to assess the influence of SBT operations on macroinvertebrates downstream of SBT outlets by estimating species diversity and pairwise community dissimilarity between upstream and downstream locations in dam-fragmented rivers with operational SBTs in comparison to dam-fragmented (i.e., no SBTs) and free-flowing rivers (i.e., no dam). We found that macroinvertebrate community dissimilarity decreases with increasing operation time and frequency of SBTs. These factors of SBT operation influence changes in riverbed features, e.g. sediment relations, that subsequently effect the recovery of downstream macroinvertebrate communities to their respective upstream communities. Macroinvertebrate abundance using morphologically-identified specimens was positively correlated to read abundance using metabarcoding. This supports and reinforces the use of quantitative estimates for diversity analysis with metabarcoding data.
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影响因子：
3.7
作者：
Elbrecht V;Leese F
通讯作者：
Leese F
DOI：
10.1007/bf00038687
发表时间：
1987-04-30
期刊：
VEGETATIO
影响因子：
--
作者：
FAITH, DP;MINCHIN, PR;BELBIN, L
通讯作者：
BELBIN, L
影响因子：
2.1
作者：
Bucklin, Ann;Lindeque, Penelope K.;Lehtiniemi, Maiju
通讯作者：
Lehtiniemi, Maiju
影响因子：
48
作者：
Edgar, Robert C.
通讯作者：
Edgar, Robert C.
影响因子：
3
作者：
Camacho C;Coulouris G;Avagyan V;Ma N;Papadopoulos J;Bealer K;Madden TL
通讯作者：
Madden TL