Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa.
Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa.
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转录组和化学分析揭示了玫瑰花颜色形成的机制
DOI：
10.3389/fpls.2022.1021521
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
玫瑰是我国著名的传统花卉，具有很高的观赏价值和良好的环境适应能力。培育丰富多彩的新种质，改善花色单调，可促进其在园林中的应用。然而，玫瑰花色形成的机理尚不清楚。本研究对具有代表性花色的玫瑰种质进行了化学分析和转录组分析。在已鉴定的花色苷中，花青素3，5-O-二葡萄糖苷(Cy3G5G)和牡丹苷3，5-O-二葡萄糖苷(Pn3G5G)是花瓣中的两个主要花色苷。Cy3G5G和Pn3G5G的总和决定了花瓣的颜色强度，如粉红色或紫色，浅红色或暗红色。Cy3G5G和Pn3G5G的比例与花瓣的色调有关，即红色或粉红色/紫色。保持高相对和高绝对含量的Cy3G5G可能是形成红色花瓣的前提。花青素的生物合成分流是花色苷积累的主要途径，这可能是导致花瓣颜色单调的主要原因，主要是粉红色/紫色。在花青素生物合成的上游途径中，35个差异表达的编码12种酶的结构基因共同表达，调节Cy3G5G和Pn3G5G的总含量，进而决定花瓣的颜色强度。RrAOMT参与花青素生物合成的下游途径，通过甲基化调节Cy3G5G和Pn3G5G的比例，进而决定花瓣的色调。值得一提的是，从深紫红色的8-16品系中检测到较高的飞雀素-3，5-O-二葡萄糖苷含量和RrF3‘5’H的表达，具有一定的独特和可见的蓝色色调。相关分析表明，3个候选转录因子RrMYB108、RrC1和RrMYB114可能通过调控RrAOMT和其他多种结构基因的表达而在花瓣颜色控制中发挥关键作用。这些结果为花色苷积累和花色机理的研究提供了新的思路，并为花色苷生物合成的候选关键基因的选育提供了有价值的资源。
Rosa rugosa is a famous Chinese traditional flower with high ornamental value and well environmental adapt ability. The cultivation of new colorful germplasms to improve monotonous flower color could promote its landscape application. However, the mechanism of flower color formation in R. rugosa remains unclear. In this study, combined analyses of the chemical and transcriptome were performed in the R. rugosa germplasms with representative flower colors. Among the identified anthocyanins, cyanidin 3,5-O-diglucoside (Cy3G5G) and peonidin 3,5-O-diglucoside (Pn3G5G) were the two dominant anthocyanins in the petals of R. rugosa. The sum content of Cy3G5G and Pn3G5G was responsible for the petal color intensity, such as pink or purple, light- or dark- red. The ratio of Cy3G5G to Pn3G5G was contributed to the petal color hue, that is, red or pink/purple. Maintaining both high relative and high absolute content of Cy3G5G may be the precondition for forming red-colored petals in R. rugosa. Cyanidin biosynthesis shunt was the dominant pathway for anthocyanin accumulation in R. rugosa, which may be the key reason for the presence of monotonous petal color in R. rugosa, mainly pink/purple. In the upstream pathway of cyanidin biosynthesis, 35 differentially expressed structural genes encoding 12 enzymes co-expressed to regulate the sum contents of Cy3G5G and Pn3G5G, and then determined the color intensity of petals. RrAOMT, involved in the downstream pathway of cyanidin biosynthesis, regulated the ratio of Cy3G5G to Pn3G5G via methylation and then determined the color hue of petals. It was worth mentioning that significantly higher delphinidin-3,5-O-diglucoside content and RrF3’5’H expression were detected from deep purple-red-flowered 8-16 germplasm with somewhat unique and visible blue hue. Three candidate key transcription factors identified by correlation analysis, RrMYB108, RrC1, and RrMYB114, might play critical roles in the control of petal color by regulating the expression of both RrAOMT and other multiple structural genes. These results provided novel insights into anthocyanin accumulation and flower coloration mechanism in R. rugosa, and the candidate key genes involved in anthocyanin biosynthesis could be valuable resources for the breeding of ornamental plants in future.
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影响因子：
3.3
作者：
Khoo HE;Azlan A;Tang ST;Lim SM
通讯作者：
Lim SM
影响因子：
7.4
作者：
Jiang, Han;Zhou, Li-Jie;Li, Yuan-Yuan
通讯作者：
Li, Yuan-Yuan
影响因子：
4.3
作者：
Lu, Wenjing;Chen, Jingxin;Luo, Zisheng
通讯作者：
Luo, Zisheng
影响因子：
5.6
作者：
Iaria DL;Chiappetta A;Muzzalupo I
通讯作者：
Muzzalupo I
崎岖玫瑰 (Rosa rugosa) 的染色体水平基因组组装提供了对其进化、生态和花卉特征的深入了解
影响因子：
8.7
作者：
Chen F;Su L;Hu S;Xue JY;Liu H;Liu G;Jiang Y;Du J;Qiao Y;Fan Y;Liu H;Yang Q;Lu W;Shao ZQ;Zhang J;Zhang L;Chen F;Cheng ZM
通讯作者：
Cheng ZM