Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis.
Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis.
复制标题
编码拟南芥电压依赖性阴离子通道的基因家族的分子和遗传表征。
DOI：
10.1093/jxb/err113
复制
发表时间：
2011-10
影响因子：
6.9
通讯作者：
Takahashi Y
中科院分区：
文献类型：
--
作者：
Tateda C;Watanabe K;Kusano T;Takahashi Y
电压依赖性阴离子通道（VDAC）是一种主要的线粒体外膜蛋白，被认为在哺乳动物系统的能量产生和细胞凋亡中发挥重要作用。然而，VDAC 在植物中的功能很大程度上未知。为了确定植物 VDAC 的个体功能，对拟南芥中发现的四个 VDAC 基因（VDAC1-VDAC4）进行了分子和遗传分析。 VDAC1 和 VDAC3 在其 C 末端具有真核线粒体孔蛋白特征 (MPS)，而 VDAC2 和 VDAC4 则不具有。 VDAC-绿色荧光蛋白 (GFP) 融合体及其嵌合或突变衍生物的定位分析表明，MPS 序列对于线粒体定位很重要。通过T-DNA插入导致的vdac敲除突变体的功能分析发现，在整个植物体中表达的VDAC2和VDAC4对于叶片发育、线粒体膜电位的稳态和花粉发育等多种生理功能具有重要意义。此外，研究表明，VDAC1 不仅是正常生长所必需的，而且通过调节过氧化氢的产生对于抗病性也很重要。
The voltage-dependent anion channel (VDAC), a major outer mitochondrial membrane protein, is thought to play an important role in energy production and apoptotic cell death in mammalian systems. However, the function of VDACs in plants is largely unknown. In order to determine the individual function of plant VDACs, molecular and genetic analysis was performed on four VDAC genes, VDAC1–VDAC4, found in Arabidopsis thaliana. VDAC1 and VDAC3 possess the eukaryotic mitochondrial porin signature (MPS) in their C-termini, while VDAC2 and VDAC4 do not. Localization analysis of VDAC–green fluorescent protein (GFP) fusions and their chimeric or mutated derivatives revealed that the MPS sequence is important for mitochondrial localization. Through the functional analysis of vdac knockout mutants due to T-DNA insertion, VDAC2 and VDAC4 which are expressed in the whole plant body are important for various physiological functions such as leaf development, the steady state of the mitochondrial membrane potential, and pollen development. Moreover, it was demonstrated that VDAC1 is not only necessary for normal growth but also important for disease resistance through regulation of hydrogen peroxide generation.
登录
查看更多内容
影响因子：
6.9
作者：
Fan, LM;Wang, YF;Wu, WH
通讯作者：
Wu, WH
DOI：
10.1016/s0167-4889(03)00102-2
发表时间：
2003-09-23
影响因子：
5.1
作者：
Godbole, A;Varghese, J;Mathew, MK
通讯作者：
Mathew, MK
影响因子：
3.9
作者：
Tsujimoto, Y;Shimizu, S
通讯作者：
Shimizu, S
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
3
作者：
Craigen, William J.;Graham, Brett H.
通讯作者：
Graham, Brett H.