The sonic hedgehog signaling pathway is suppressed following PCB1254 exposure during retinal development
The sonic hedgehog signaling pathway is suppressed following PCB1254 exposure during retinal development
复制标题
视网膜发育过程中暴露于 PCB1254 后，声波刺猬信号通路受到抑制
DOI：
10.1002/tox.22689
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发表时间：
2019-03
影响因子：
4.5
通讯作者：
Qinghuai Liu
中科院分区：
文献类型：
--
作者：
Ning Wei;Xin Zhang;Qin Hong;Yue Jiang;Qinyu Zhang;Xirong Zhang;Chi Xia;Meiling Tong;Qinghuai Liu
据报道，多氯联苯（PCB）对视网膜发育有不利影响。为了探讨Shh信号在体内和体外暴露PCB1254后视网膜发育中的作用，我们使用斑马鱼和RGC-5视网膜细胞系。与对照组相比，多氯联苯暴露抑制细胞增殖，增加细胞凋亡水平。与对照组相比，体内和体外pcb1254处理组Shh mRNA的表达均下降。实验组ptch2 mRNA表达升高。pcb1254处理组gli2 mRNA表达降低。免疫荧光和western blotting检测证实，与对照组相比，pcb1254处理组Shh蛋白的表达降低。此外，pcb1254处理组ptch2蛋白水平升高，gli1和gli2蛋白表达降低。这些结果表明Shh信号通路可能参与了PCB1254暴露导致的视网膜发育损伤，为环境污染物可能引起眼病提供了证据。
Polychlorinated biphenyl (PCB) has been reported to have detrimental effects on retinal development. In order to explore the role of Shh signaling in retinal development after PCB1254 exposure in vivo and in vitro, zebrafish and RGC-5 retinal cell line were used. Compared with the controls, PCB exposure inhibited proliferation and increased the apoptosis levels. The expression of Shh mRNA decreased in the PCB1254-treated groups both in vivo and in vitro compared with that of the controls. The ptch2 mRNA expression increased in the experimental groups. The expression of gli2 mRNA decreased in the PCB1254-treated groups. Immunofluorescence and western blotting assays confirmed that the expression of Shh proteins decreased in PCB1254-treated groups compared with control groups. Moreover, ptch2 protein levels increased in the PCB1254-treated groups as well as the decreased protein expressions of gli1and gli2. These results demonstrated that Shh signaling pathway may participate in the damage of retinal development caused by PCB1254 exposure, providing evidence that eye diseases could be caused by environmental pollutants.
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影响因子：
4.7
作者：
Pi LH;Chen L;Liu Q;Ke N;Fang J;Zhang S;Xiao J;Ye WJ;Xiong Y;Shi H;Zhou XY;Yin ZQ
通讯作者：
Yin ZQ
影响因子：
7.7
作者：
Alena Shkumatava;C. Neumann
通讯作者：
Alena Shkumatava;C. Neumann
影响因子：
4.6
作者：
A. Jensen;Valerie A. Wallace
通讯作者：
A. Jensen;Valerie A. Wallace
影响因子：
4.6
作者：
P. Haffter;M. Granato;M. Brand;M. Mullins;M. Hammerschmidt;D. Kane;J. Odenthal;F. V. Eeden;Yun-Jin Jiang;C. Heisenberg;R. Kelsh;M. Furutani-Seiki;E. Vogelsang;D. Beuchle;Ursula Schach;Cosima Fabian;C. Nüsslein-Volhard
通讯作者：
P. Haffter;M. Granato;M. Brand;M. Mullins;M. Hammerschmidt;D. Kane;J. Odenthal;F. V. Eeden;Yun-Jin Jiang;C. Heisenberg;R. Kelsh;M. Furutani-Seiki;E. Vogelsang;D. Beuchle;Ursula Schach;Cosima Fabian;C. Nüsslein-Volhard
影响因子：
4.6
作者：
K. Larison;R. Bremiller
通讯作者：
K. Larison;R. Bremiller