Interleukin 10 and transforming growth factor beta cooperate to induce anti-CD40-activated naive human B cells to secrete immunoglobulin A.
Interleukin 10 and transforming growth factor beta cooperate to induce anti-CD40-activated naive human B cells to secrete immunoglobulin A.
复制标题
DOI：
10.1084/jem.175.3.671
复制
发表时间：
1992-03-01
影响因子：
15.3
通讯作者：
BANCHEREAU, J
中科院分区：
文献类型：
--
作者：
DEFRANCE, T;VANBERVLIET, B;BRIERE, F;DURAND, I;ROUSSET, F;BANCHEREAU, J
在本报告中，我们研究了表面 sIgD+ 和 sIgD- 人类 B 细胞响应各种刺激而体外分化为 Ig 分泌细胞的情况。 sIgD+ B 细胞均匀表达一些识别套区 B 细胞的抗原，但缺乏生发中心标志物的表达，从而证实基于 sIgD 表达阳性选择的 B 细胞群高度富集初始 B 淋巴细胞。相反，sIgD-B 细胞表达一些与生发中心 B 细胞特异性相关的抗原。 sIgD+ 和 sIgD- B 细胞的 T 细胞依赖性分化可以通过在稳定表达人 CDw32/Fc gamma RII（CDw32 L 细胞）的小鼠 Ltk-细胞系存在下同时交联 sIgs 和 CD40 来实现。在该实验系统中，sIgD+ B 细胞专门用于 IgM 合成，而 sIgD- B 细胞则产生 IgG、IgM 和 IgA。人和病毒形式的白细胞介素 10 (IL-10) 均强烈增加通过 sIgs 和 CD40 同时激活的 sIgD+ 和 sIgD- B 细胞的 Ig 分泌。 IgM 和 IgG 分别构成 sIgD+ 和 sIgD- B 细胞响应 IL-10 产生的主要 Ig 同种型。在 CDw32 L 细胞呈递的抗 CD40 单克隆抗体存在的情况下，与转化生长因子 β (TGF-β) 和 IL-10 共培养，可诱导 sIgD+ B 细胞合成 IgA。相反，TGF-β抑制sIgD-B细胞IL-10介导的IgG、IgM和IgA分泌。 sIgD+ B 细胞在 sIgs 交联后，不能被 TGF-β 和 IL-10 诱导合成 IgA，这表明 CD40 的连接是初始 B 细胞分泌 IgA 所需的必需信号之一。有限稀释实验表明，TGF-β 的 IgA 增强作用是由于其能够增加 IgA 产生细胞的频率，这很可能是类别转换的结果。总而言之，我们的数据强烈表明 TGF-β 参与人类 IgA 同种型选择的调节。
In the present report, we have investigated the in vitro differentiation of surface(s) sIgD+ and sIgD- human B cells into Ig- secreting cells in response to various stimuli. sIgD+ B cells homogeneously expressed some of the antigens identifying mantle zone B cells, but lacked expression of germinal center markers, thus confirming that the B cell populations positively selected on the basis of sIgD expression were highly enriched for naive B lymphocytes. Conversely, sIgD- B cells expressed some of the antigens specifically associated with germinal center B cells. T cell-independent differentiation of sIgD+ and sIgD- B cells could be achieved by simultaneous crosslinking of sIgs and CD40 in the presence of a mouse Ltk- cell line stably expressing human CDw32/Fc gamma RII (CDw32 L cells). In this experimental system, sIgD+ B cells were exclusively proned for IgM synthesis, whereas sIgD- B cells produced IgG, IgM, and IgA. Both the human and viral forms of interleukin 10 (IL-10) strongly increased the Ig secretion by sIgD+ and sIgD- B cells simultaneously activated through sIgs and CD40. IgM and IgG constituted the predominant Ig isotype produced by sIgD+ and sIgD- B cells, respectively, in response to IL-10. sIgD+ B cells could be induced for IgA synthesis upon co-culturing with transforming growth factor beta (TGF-beta) and IL-10, in the presence of an anti-CD40 monoclonal antibody presented by the CDw32 L cells. In contrast, TGF-beta suppressed the IL-10-mediated IgG, IgM, and IgA secretions by sIgD- B cells. sIgD+ B cells could not be induced for IgA synthesis by TGF-beta and IL-10 after crosslinking of their sIgs, suggesting that ligation of CD40 was one of the obligatory signals required for commitment of naive B cells to IgA secretion. Limiting dilution experiments indicated that the IgA-potentiating effect of TGF-beta was due to its capacity to increase the frequency of IgA-producing cells, most likely as a consequence of class switching. Taken together, our data strongly suggest that TGF-beta is involved in the regulation of IgA isotype selection in humans.
登录
查看更多内容
DOI：
10.1084/jem.170.6.2011
发表时间：
1989-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Mourad W;Scholl P;Diaz A;Geha R;Chatila T
通讯作者：
Chatila T
DOI：
10.1084/jem.173.3.747
发表时间：
1991-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gascan H;Gauchat JF;Roncarolo MG;Yssel H;Spits H;de Vries JE
通讯作者：
de Vries JE
影响因子：
56.9
作者：
BANCHEREAU, J;DEPAOLI, P;ROUSSET, F
通讯作者：
ROUSSET, F
CD40和IGE：抗CD40单克隆抗体和白介素4之间的协同作用在高度纯化的人B细胞诱导IgE合成中。
影响因子：
15.3
作者：
Jabara, H H;Fu, S M;Geha, R S;Vercelli, D
通讯作者：
Vercelli, D
影响因子：
5.4
作者：
MIYAWAKI, T;BUTLER, JL;COOPER, MD
通讯作者：
COOPER, MD