Genotypic analysis of two hypervariable human cytomegalovirus genes.
Genotypic analysis of two hypervariable human cytomegalovirus genes.
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DOI：
10.1002/jmv.21241
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发表时间：
2008-09
影响因子：
12.7
通讯作者：
Davison, Andrew J.
中科院分区：
文献类型：
--
作者：
Bradley, Amanda J.;Kovacs, Ida J.;Gatherer, Derek;Dargan, Derrick J.;Alkharsah, Khaled R.;Chan, Paul K. S.;Carman, William F.;Dedicoat, Martin;Emery, Vincent C.;Geddes, Colin C.;Gerna, Giuseppe;Ben-Ismaeil, Bassam;Kaye, Steve;McGregor, Alistair;Moss, Paul A.;Pusztai, Rozalia;Rawlinson, William D.;Scott, Gillian M.;Wilkinson, Gavin W. G.;Schulz, Thomas F.;Davison, Andrew J.
大多数人类巨细胞病毒（HCMV）基因在菌株之间的序列高度保守，但有些基因表现出相当程度的变异。其中两个基因是UL146和UL139，前者编码一种CXC趋化因子，后者预计编码一种膜糖蛋白。这些基因的序列是从来自非洲、澳大利亚、亚洲、欧洲和北美的184份HCMV样本中确定的。UL146在整个过程中都是高可变的，而UL139的变异集中在编码可能高度糖基化区域的序列中。与之前报道的序列一样，UL146序列也可分为14个基因型。UL139序列分为8个基因型，所有先前报道的序列都属于这些基因型的一个子集。UL146和UL139的基因型频率在各大洲间存在较小差异，但没有明显的地理分离，在相距较远的群落中具有相同的核苷酸序列。多基因型的频繁检测表明混合感染是常见的。对于这两个基因，差异的程度足以排除在大多数可变区域的基因型之间可靠的序列比对，并且无法辨别产生基因型的进化模式。在基因型中，约束似乎是主要的模式，阳性选择最多被检测到。没有发现连杆不平衡的证据。新出现的情况是，HCMV基因型在早期（甚至更早）人群中发展，通过创始人效应或瓶颈效应建立起来，并在最近的时代在全球范围内传播、重组和混合。
Most human cytomegalovirus (HCMV) genes are highly conserved in sequence among strains, but some exhibit a substantial degree of variation. Two of these genes are UL146, which encodes a CXC chemokine, and UL139, which is predicted to encode a membrane glycoprotein. The sequences of these genes were determined from a collection of 184 HCMV samples obtained from Africa, Australia, Asia, Europe, and North America. UL146 is hypervariable throughout, whereas variation in UL139 is concentrated in a sequence encoding a potentially highly glycosylated region. The UL146 sequences fell into 14 genotypes, as did all previously reported sequences. The UL139 sequences grouped into 8 genotypes, and all previously reported sequences fell into a subset of these. There were minor differences among continents in genotypic frequencies for UL146 and UL139, but no clear geographical separation, and identical nucleotide sequences were represented among communities distant from each other. The frequent detection of multiple genotypes indicated that mixed infections are common. For both genes, the degree of divergence was sufficient to preclude reliable sequence alignments between genotypes in the most variable regions, and the mode of evolution involved in generating the genotypes could not be discerned. Within genotypes, constraint appears to have been the predominant mode, and positive selection was detected marginally at best. No evidence was found for linkage disequilibrium. The emerging scenario is that the HCMV genotypes developed in early human populations (or even earlier), becoming established via founder or bottleneck effects, and have spread, recombined and mixed worldwide in more recent times.
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影响因子：
12.7
作者：
Poncet, C;Frances, V;FigarellaBranger, D
通讯作者：
FigarellaBranger, D
影响因子：
3.8
作者：
Pignatelli, S;Dal Monte, P;Landini, MP
通讯作者：
Landini, MP
影响因子：
3.8
作者：
Davison, AJ;Dolan, A;Hayward, GS
通讯作者：
Hayward, GS
影响因子：
8.8
作者：
Iwasenko, Jenna M.;Scott, Gillian A.;Rawlinson, William D.
通讯作者：
Rawlinson, William D.
影响因子：
3.8
作者：
Pignatelli, S;Dal Monte, P;Landini, MP
通讯作者：
Landini, MP