Surface oxide net charge of a titanium alloy: modulation of fibronectin-activated attachment and spreading of osteogenic cells.
Surface oxide net charge of a titanium alloy: modulation of fibronectin-activated attachment and spreading of osteogenic cells.
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DOI：
10.1016/j.colsurfb.2010.08.023
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发表时间：
2011-01-01
影响因子：
5.8
通讯作者：
MacDonald, Daniel E.
中科院分区：
文献类型：
--
作者：
Rapuano, Bruce E.;MacDonald, Daniel E.
在当前的研究中，我们通过热处理或射频辉光放电（RFGD）改变了Ti6Al4V合金的表面氧化物性能，以评估合金表面氧化物的物理化学和生物性能之间的关系。研究了表面预处理对两种成骨细胞系（MG63和MC3T3）和间充质干细胞（C3H10T1/2）吸附在合金上的纤维连接蛋白附着的影响。对于每个被测试的细胞系，热和RFGD预处理都使附着在合金上的纤维连接蛋白的细胞数量增加了几倍（0.001和10 nM FN）。一种针对纤维连接蛋白中央整合素结合域的抗体（HFN7.1）可抑制65-70%的细胞对纤维连接蛋白涂覆的金属盘的附着，表明细胞对金属盘的附着依赖于纤维连接蛋白与细胞整合素受体的结合。两种处理都加速了细胞扩散反应，表现为广泛的变平和平均细胞面积的增加。处理诱导的吸附纤维连接蛋白细胞附着活性的增加与先前证明的高温和RFGD预处理在生理pH下产生的Ti6Al4V氧化物负净表面电荷的增加有关。由于两种处理都没有增加纤维连接蛋白的吸附量，这些发现表明，Ti6Al4V中带负电荷的表面氧化官能团可以通过改变吸附蛋白的构象来调节纤维连接蛋白的整合素受体活性。我们的研究结果进一步表明，表面氧化物中带负电荷的官能团在金属种植材料的骨整合中起着重要作用。
In the current study, we have altered the surface oxide properties of a Ti6Al4V alloy using heat treatment or radiofrequency glow discharge (RFGD) in order to evaluate the relationship between the physico-chemical and biological properties of the alloy's surface oxide. The effects of surface pretreatments on the attachment of cells from two osteogenic cell lines (MG63 and MC3T3) and a mesenchymal stem cell line (C3H10T1/2) to fibronectin adsorbed to the alloy were measured. Both heat and RFGD pretreatments produced a several-fold increase in the number of cells that attached to fibronectin adsorbed to the alloy (0.001 and 10 nM FN) for each cell line tested. An antibody (HFN7.1) directed against the central integrin binding domain of fibronectin produced a 65-70% inhibition of cell attachment to fibronectin-coated disks, incdicating that cell attachment to the metal discs was dependent on fibronectin binding to cell integrin receptors. Both treatments also accelerated the cell spreading response manifested by extensive flattening and an increase in mean cellular area. The treatment-induced increases in the cell attachment activity of adsorbed fibronectin were correlated with previously demonstrated increases in Ti6Al4V oxide negative net surface charge at physiological pH produced by both heat and RFGD pretreatments. Since neither treatment increased the adsorption mass of fibronectin, these findings suggest that negatively charged surface oxide functional groups in Ti6Al4V can modulate fibronectin's integrin receptor activity by altering the adsorbed protein's conformation. Our results further suggest that negatively charged functional groups in the surface oxide can play a prominent role in the osseointegration of metallic implant materials.
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DOI：
10.1016/s1389-0344(02)00032-1
发表时间：
2002-08-01
期刊：
BIOMOLECULAR ENGINEERING
影响因子：
--
作者：
Eisenbarth, E;Velten, D;Hildebrand, H
通讯作者：
Hildebrand, H
影响因子：
4.9
作者：
Baugh, L;Vogel, V
通讯作者：
Vogel, V
影响因子：
3.7
作者：
ALTANKOV, G;GROTH, T
通讯作者：
GROTH, T
影响因子：
2.7
作者：
Gotman, I
通讯作者：
Gotman, I
影响因子：
14
作者：
Anselme, K;Linez, P;Leroy, JM
通讯作者：
Leroy, JM