Roles of natural resistance-associated macrophage protein-1 in modulating bacterial distribution and immune responses during Salmonella enterica serovar Typhimurium infection in murine pregnancy.
Roles of natural resistance-associated macrophage protein-1 in modulating bacterial distribution and immune responses during Salmonella enterica serovar Typhimurium infection in murine pregnancy.
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DOI：
10.1111/aji.13599
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发表时间：
2022-10
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影响因子：
--
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中科院分区：
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鼠伤寒沙门氏菌(S.Tm)在妊娠期间感染Nramp1+/+小鼠可导致胎盘单位细菌大量生长，并导致不良妊娠结局，包括胎儿丧失、产妇疾病和死亡。金黄色葡萄球菌进入胎儿-胎盘单位，并通过组织传播导致胎盘吸收和胎儿死亡的动力学和机制尚不清楚。小鼠全身感染S.Tm。对脾和单个胎盘内的细菌负荷以及胎盘/胎儿的吸收进行了量化。对脾和单个胎盘的免疫细胞类型进行了流式细胞术分析。采用流式细胞仪检测母血中细胞因子的表达。与Nramp1+/+小鼠的脾相比，全身感染S.Tm导致胎盘细菌优先增殖。感染后24小时，每只小鼠单个胎盘的平均感染率为50%，到感染后72小时，单个胎盘感染率上升至75%，提示少数部位的初始感染可通过子宫环境快速传播。这与胎盘/胎儿吸收率的稳步上升有关。胎盘感染与局部中性粒细胞百分比的增加有关，而脾中的数量和百分比保持不变，这表明全身间隙和母胎界面之间的炎症调节是二元的。感染期间怀孕小鼠存活率的降低与血清干扰素-γ的降低有关，但与非妊娠对照组相比IL-10水平的升高有关。怀孕通过母体和胎盘细胞反应的间隔性调节和系统细胞因子表达的调节，损害了Nramp1对S.Tm的宿主抵抗。
Salmonella enterica serovar Typhimurium (S.Tm) infection in Nramp1+/+ mice during pregnancy can lead to profound bacterial growth in the feto-placental unit and adverse pregnancy outcomes, including fetal loss, maternal illness and death. The kinetics and mechanisms by which S.Tm gains entry within individual feto-placental unit, and disseminates through tissues leading to placental resorption and fetal demise remain unclear. Mice were systemically infected with S.Tm. Bacterial burden within spleen and individual placentas, and placental/fetal resorptions were quantified. Flow cytometric analysis of immune cell types in the spleen and individual placentas was performed. Cytokine expression in maternal serum was determined through cytometric bead array. Systemic infection with S.Tm resulted in preferential bacterial proliferation in placentas compared to the spleen in Nramp1+/+ mice. At 24h post-infection, the mean infection rate of individual placentas per mouse was ~50%, increasing to >75% by 72h post-infection, suggesting that initial infection in few sites progresses to rapid spread of infection through the uterine milieu. This correlated with a steady increase in placental/fetal resorption rates. Placental infection was associated with local increased neutrophil percentages, whereas numbers and percentages in the spleen remained unchanged, suggesting dichotomous modulation of inflammation between the systemic compartment and the feto-maternal interface. Reduced survival rates of pregnant mice during infection correlated with decreased serum IFN-γ but increased IL-10 levels relative to non-pregnant controls. Pregnancy compromises host resistance conferred by Nramp1 against S.Tm through compartment-specific regulation of maternal and placental cellular responses, and modulation of systemic cytokine expression.
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影响因子：
5.2
作者：
Mor G;Cardenas I;Abrahams V;Guller S
通讯作者：
Guller S
影响因子：
4.4
作者：
Pejcic-Karapetrovic, Branka;Gurnani, Komal;Krishnan, Laksluni
通讯作者：
Krishnan, Laksluni
影响因子：
4.4
作者：
Kurtz JR;Goggins JA;McLachlan JB
通讯作者：
McLachlan JB
影响因子：
64.5
作者：
Arpaia N;Godec J;Lau L;Sivick KE;McLaughlin LM;Jones MB;Dracheva T;Peterson SN;Monack DM;Barton GM
通讯作者：
Barton GM
DOI：
10.1111/j.1600-0897.2010.00836.x
发表时间：
2010-06
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Mor G;Cardenas I
通讯作者：
Cardenas I