Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis.
Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis.
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自动化的微流体设备集成了固相提取，荧光标记和微芯片电泳，以进行早产生物标志物分析。
DOI：
10.1007/s00216-017-0548-7
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发表时间：
2018-01
影响因子：
4.3
通讯作者：
Woolley AT
中科院分区：
文献类型：
--
作者：
Sahore V;Sonker M;Nielsen AV;Knob R;Kumar S;Woolley AT
我们开发了多通道集成微流体装置，用于早产（PTB）生物标志物的自动预浓缩，标记，纯化和分离。我们制作了多层聚（二甲基硅氧烷）-环烯烃共聚物（PDMS-COC）装置，可执行固相萃取（SPE）和微芯片电泳（μCE），用于自动化PTB生物标志物分析。PDMS控制层具有用于流量控制的蠕动泵和气动阀，而PDMS流体层具有连接到微通道和μCE系统的五个输入储液器。COC层具有用于SPE和PTB生物标志物的荧光标记的反相甲基丙烯酸辛酯多孔聚合物整料。我们确定了两种PTB生物标志物铁蛋白（Fer）和促肾上腺皮质激素释放因子（CRF）的μCE条件。我们使用这些集成的微流控装置以自动化的方式预浓缩和纯化芯片外标记的Fer和CRF。最后，我们对未标记的PTB生物标志物进行了全自动芯片分析，包括SPE，标记和μCE分离，总分析时间为1小时。这些集成系统具有与上游免疫亲和提取相结合的强大潜力，提供了一个紧凑的样品到答案的生物标志物分析平台。
We have developed multichannel integrated microfluidic devices for automated preconcentration, labeling, purification and separation of preterm birth (PTB) biomarkers. We fabricated multi-layer poly(dimethylsiloxane)-cyclic olefin copolymer (PDMS-COC) devices, that perform solid-phase extraction (SPE) and microchip electrophoresis (μCE) for automated PTB biomarker analysis. The PDMS control layer had a peristaltic pump and pneumatic valves for flow control, while the PDMS fluidic layer had five input reservoirs connected to microchannels and a μCE system. The COC layers had a reversed-phase octyl methacrylate porous polymer monolith for SPE and fluorescent labeling of PTB biomarkers. We determined μCE conditions for two PTB biomarkers, ferritin (Fer) and corticotropin releasing factor (CRF). We used these integrated microfluidic devices to preconcentrate and purify off-chip-labeled Fer and CRF in an automated fashion. Finally, we performed a fully automated on-chip analysis of unlabeled PTB biomarkers, involving SPE, labeling and μCE separation with 1 hour total analysis time. These integrated systems have strong potential to be combined with upstream immunoaffinity extraction, offering a compact sample-to-answer biomarker analysis platform.
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影响因子：
9.8
作者：
Esplin MS;Merrell K;Goldenberg R;Lai Y;Iams JD;Mercer B;Spong CY;Miodovnik M;Simhan HN;van Dorsten P;Dombrowski M;Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
通讯作者：
Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
影响因子：
62.1
作者：
Nge, Pamela N.;Rogers, Chad I.;Woolley, Adam T.
通讯作者：
Woolley, Adam T.
影响因子：
2.9
作者：
Sun, Xuefei;Kelly, Ryan T.;Smith, Richard D.
通讯作者：
Smith, Richard D.
影响因子：
7.4
作者：
Yang, Mian;Nelson, Randall;Ros, Alexandra
通讯作者：
Ros, Alexandra
影响因子：
10.8
作者：
Krivitsky, Vadim;Hsiung, Lo-Chang;Patolsky, Fernando
通讯作者：
Patolsky, Fernando