Relaxin enhances bone regeneration with BMP-2-loaded hydroxyapatite microspheres.
Relaxin enhances bone regeneration with BMP-2-loaded hydroxyapatite microspheres.
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DOI：
10.1002/jbm.a.36897
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发表时间：
2020-05
期刊：
影响因子：
--
通讯作者：
Huang YW
中科院分区：
文献类型：
--
作者：
Injamuri S;Rahaman MN;Shen Y;Huang YW
我们的目的是：1)评价中空羟基磷灰石微球(2 12~2 5 0μm)作为骨形态发生蛋白-2体外控释载体的能力；2)检测松弛素作为骨形态发生蛋白-2的骨再生促进剂。将硼酸盐玻璃微球转化为HA空心微球，用X射线衍射、傅里叶变换红外光谱、扫描电子显微镜和Brunauer-Emmett-Teller方法对其进行了表征。将骨形态发生蛋白-2和松弛素复合微球植入SD大鼠颅骨缺损区，共植入6周。骨形态发生蛋白-2在每个缺损区1μg的范围内单独显示出剂量依赖性的骨再生，而松弛素单用在每个缺损区0.25μg的范围内不能促进骨再生。与单独使用骨形态发生蛋白-2(每个缺损区1μg)相比，每个缺损区添加0.0 5、0.1或0.2 5μg松弛素组的BMP-2剂量减少了5 0%。这些结果表明，负载松弛素和BMP-2的HA微球可以显著减少再生等量骨所需的BMP-2剂量。
Our aims were to 1) evaluate the capacity of hollow hydroxyapatite (HA) microspheres (212–250 μm) to serve as a delivery system for controlled release of BMP-2 in vitro and 2) examine relaxin as an enhancer of BMP-2 for bone regeneration. Hollow HA microspheres were converted from borate glass microspheres and characterized using X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, and the Brunauer–Emmett–Teller method. The microspheres loaded with BMP-2 and relaxin were implanted for 6 weeks in Sprague Dawley rats with calvarial defects. BMP-2 alone in the range up to 1 μg per defect exhibited dose-dependent bone regeneration while relaxin alone in the range up to 0.25 μg per defect did not promote bone regeneration. When compared with BMP-2 alone (1 μg per defect), a 50% reduction in the BMP-2 dose was achieved with the addition of 0.05, 0.1, or 0.25 μg of relaxin per defect. These results show that loading HA microspheres with a combination of relaxin and BMP-2 can significantly reduce the BMP-2 dose required to regenerate an equivalent amount of bone.
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影响因子：
14
作者：
Lee, Sungsoo S.;Huang, Brian J.;Kaltz, Stuart R.;Sur, Shantanu;Newcomb, Christina J.;Stock, Stuart R.;Shah, Ramille N.;Stupp, Samuel I.
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作者：
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