Adenosine triphosphate activates ion permeabilities in biliary epithelial cells.
Adenosine triphosphate activates ion permeabilities in biliary epithelial cells.
复制标题
三磷酸腺苷激活胆管上皮细胞的离子渗透性。
DOI：
10.1016/0016-5085(94)90082-5
复制
发表时间：
1994
期刊：
影响因子：
29.4
通讯作者：
Fitz,JG
中科院分区：
文献类型：
--
作者：
McGill,JM;Basavappa,S;Mangel,AW;Shimokura,GH;Middleton,JP;Fitz,JG
背景/目的：胆管上皮通过吸收和分泌液体和电解质促进胆汁的形成。方法：用125I和86Rb的外流率分别测定胆管细胞膜CI、−和K+通透性。结果：嘌呤能受体激动剂ATP和尿苷三磷酸可刺激125I和86Rb外流，约为基础水平的两倍。腺苷及其受体激动剂5‘-N-乙基氨基腺苷、N6-(2-苯基异丙基)腺苷也能刺激125I的外流，这些作用可被黄嘌呤胺类化合物所抑制。ATP、腺苷5‘-O-(3-硫代磷酸)和尿苷三磷酸均可刺激细胞内钙释放，而腺苷则无此作用。在Mz-CHA-1细胞的全细胞记录中，三磷酸腺苷激活了与K+电导一致的早期瞬时外向电流和与CI−电导一致的持续内向电流。结论：胆管细胞至少存在两种核苷酸受体，通过钙依赖和非依赖途径调节膜离子通透性，三磷酸腺苷可能参与胆汁分泌的调节。
Background/Aims:The biliary epithelium contributes to bile formation through absorption and secretion of fluid and electrolytes. The effects of extracellular nucleotides on membrane ion transport were assessed in isolated bile duct cells from rats and Mz-ChA-1 cells from a human cholangiocarcinoma.Methods:The rates of efflux of125I and86Rb were used to assess membrane CI−and K+permeabilities, respectively. Patch clamp recordings of whole cell currents were used to evaluate the properties of adenosine triphosphate (ATP)-activated currents.Results:Purinergic receptor agonists ATP and uridine triphosphate stimulated125I and86Rb efflux about twofold above basal levels. The effects were reproduced by a nonhydrolyzable analogue of ATP (adenosine 5′-O-[3-thiophosphate]) and were unaffected by an adenosine receptor blocker xanthine amine congener.125I efflux was also stimulated by adenosine and its receptor agonists 5′-N-ethylcar-boxamidoadenosine,N6-(2-phenylisopropyl)adenosine; these effects were inhibited by xanthine amine congener, suggesting a separate adenosine receptor. ATP, adenosine 5′-O-(3-thiophosphate), and uridine triphosphate each stimulated release of Ca2+from intracellular stores, whereas adenosine had no effect. In whole cell recordings of Mz-ChA-1 cells, ATP activated an early transient outward current consistent with a K+conductance and a later, sustained inward current consistent with a CI−conductance.Conclusions:Biliary cells possess at least two classes of nucleotide receptors that modulate membrane ion permeability through Ca2+-dependent and -independent pathways, and ATP may be involved in the regulation of biliary secretion.
登录
查看更多内容
细胞外 ATP 在 T51B 大鼠肝上皮细胞中动员细胞内 Ca2：一项涉及单细胞测量的研究。
影响因子：
3.7
作者：
Boynton,AL;Cooney,RV;Hill,TD;Nilsson,T;Arkhammar,P;Berggren,PO
通讯作者：
Berggren,PO
影响因子：
29.4
作者：
BASAVAPPA, S;MIDDLETON, J;FITZ, JG
通讯作者：
FITZ, JG
概述：嘌呤能机制
影响因子：
5.2
作者：
G. Burnstock
通讯作者：
G. Burnstock
促胰液素通过环 AMP 介导的机制刺激离体胆管上皮细胞的胞吐作用。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kato,A;Gores,GJ;LaRusso,NF
通讯作者：
LaRusso,NF
胆囊粘膜功能：人类和狗胆囊上皮吸收和分泌的研究。
DOI：
10.1152/ajpgi.1992.263.1.g69
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Igimi,H;Yamamoto,F;Lee,SP
通讯作者：
Lee,SP