Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics.
Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics.
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DOI：
10.1039/c6mb00239k
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发表时间：
2016-08-16
影响因子：
--
通讯作者：
David Eckersall P
中科院分区：
文献类型：
--
作者：
Thomas FC;Mullen W;Tassi R;Ramírez-Torres A;Mudaliar M;McNeilly TN;Zadoks RN;Burchmore R;David Eckersall P
急性期蛋白质和一组77肽的生物标志物面板增加牛奶在奶牛乳腺炎引起的链球菌感染的乳腺。对奶牛链球菌乳腺炎实验模型乳汁的肽组研究纳入了乳汁高丰度和急性期（APP）蛋白的研究以及低分子量肽生物标志物的分析。乳房内感染（IMI）与S。乳铁蛋白引起从酪蛋白、β-乳球蛋白和α-乳白蛋白到白蛋白、乳铁蛋白和IgG的丰度变化，乳铁蛋白的增加最后发生。结合珠蛋白、乳腺相关血清淀粉样蛋白A3和C-反应蛋白的APP反应发生在攻毒后30-48小时之间，APP的峰值浓度在攻毒后72-96小时，此后以类似于细菌计数而不是体细胞计数下降的速率下降。建立了一种基于毛细管电泳和质谱的IMI肽生物标志物检测板。它包括77个鉴定的肽（IMI 77），主要由酪蛋白衍生肽组成，但也包括糖基化依赖性细胞粘附分子和血清淀粉样蛋白A的肽。该组的生物标志物分类评分从攻毒后36小时增加到81小时，显著区分了感染的乳汁和未感染的乳汁，因此表明其有潜力作为牛乳腺炎的肽生物标志物组，特别是S.可持续发展的起源组学技术的使用显示了高丰度和低丰度蛋白质及其肽衍生物的多因子交叉系统反应，响应于S.尿路感染
Acute phase proteins and a group of 77 peptides in a biomarker panel increase in milk during bovine mastitis caused by a Streptococcus uberis infection of mammary glands. A peptidomic investigation of milk from an experimental model of Streptococcus uberis mastitis in dairy cows has incorporated a study of milk high abundance and acute phase (APP) proteins as well as analysis of low molecular weight peptide biomarkers. Intramammary infection (IMI) with S. uberis caused a shift in abundance from caseins, β-lactoglobulin and α-lactalbumin to albumin, lactoferrin and IgG with the increase in lactoferrin occurring last. The APP response of haptoglobin, mammary associated serum amyloid A3 and C-reactive protein occurred between 30–48 hours post challenge with peak concentrations of APPs at 72–96 hours post challenge and declined thereafter at a rate resembling the fall in bacterial count rather than the somatic cell count. A peptide biomarker panel for IMI based on capillary electrophoresis and mass spectrometry was developed. It comprised 77 identified peptides (IMI77) composed mainly of casein derived peptides but also including peptides of glycosylation dependent cell adhesion molecule and serum amyloid A. The panel had a biomarker classification score that increased from 36 hour to 81 hour post challenge, significantly differentiating infected from non-infected milk, thus suggesting potential as a peptide biomarker panel of bovine mastitis and specifically that of S. uberis origin. The use of omic technology has shown a multifactorial cross system reaction in high and low abundance proteins and their peptide derivatives with changes of over a thousand fold in analyte levels in response to S. uberis infection.
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影响因子：
3.3
作者：
Braceland M;Bickerdike R;Tinsley J;Cockerill D;Mcloughlin MF;Graham DA;Burchmore RJ;Weir W;Wallace C;Eckersall PD
通讯作者：
Eckersall PD
影响因子：
3.3
作者：
Bannerman, D. D.
通讯作者：
Bannerman, D. D.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.4
作者：
Bannerman, DD;Paape, MJ;Hope, JC
通讯作者：
Hope, JC
影响因子：
7
作者：
Good, David M.;Zuerbig, Petra;Schmitt-Kopplin, Philippe
通讯作者：
Schmitt-Kopplin, Philippe