A label-free microfluidic biosensor for activity detection of single microalgae cells based on chlorophyll fluorescence.
A label-free microfluidic biosensor for activity detection of single microalgae cells based on chlorophyll fluorescence.
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基于叶绿素荧光的无标记微流控生物传感器用于单个微藻细胞活性检测
DOI：
10.3390/s131216075
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发表时间：
2013-11-26
期刊：
影响因子：
--
通讯作者：
Li D
中科院分区：
文献类型：
--
作者：
Wang J;Sun J;Song Y;Xu Y;Pan X;Sun Y;Li D
Detection of living microalgae cells is very important for ballast water treatment and analysis. Chlorophyll fluorescence is an indicator of photosynthetic activity and hence the living status of plant cells. In this paper, we developed a novel microfluidic biosensor system that can quickly and accurately detect the viability of single microalgae cells based on chlorophyll fluorescence. The system is composed of a laser diode as an excitation light source, a photodiode detector, a signal analysis circuit, and a microfluidic chip as a microalgae cell transportation platform. To demonstrate the utility of this system, six different living and dead algae samples (Karenia mikimotoi Hansen, Chlorella vulgaris, Nitzschia closterium, Platymonas subcordiformis, Pyramidomonas delicatula and Dunaliella salina) were tested. The developed biosensor can distinguish clearly between the living microalgae cells and the dead microalgae cells. The smallest microalgae cells that can be detected by using this biosensor are 3 μm ones. Even smaller microalgae cells could be detected by increasing the excitation light power. The developed microfluidic biosensor has great potential for in situ ballast water analysis.
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影响因子：
12.6
作者：
Chikkaveeraiah, Bhaskara V.;Mani, Vigneshwaran;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
3.9
作者：
Mathur, S.;Jajoo, A.;Bharti, S.
通讯作者：
Bharti, S.
影响因子：
4.2
作者：
Else, Mark A.;Janowiak, Franciszek;Jackson, Michael B.
通讯作者：
Jackson, Michael B.
DOI：
10.3390/s120911853
发表时间：
2012
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Fernandez-Jaramillo AA;Duarte-Galvan C;Contreras-Medina LM;Torres-Pacheco I;de J Romero-Troncoso R;Guevara-Gonzalez RG;Millan-Almaraz JR
通讯作者：
Millan-Almaraz JR
影响因子：
8.4
作者：
Grover, WH;Skelley, AM;Mathies, RA
通讯作者：
Mathies, RA