Mago Nashi, Tsunagi/Y14, and Ranshi form a complex that influences oocyte differentiation in Drosophila melanogaster.
Mago Nashi, Tsunagi/Y14, and Ranshi form a complex that influences oocyte differentiation in Drosophila melanogaster.
复制标题
DOI：
10.1016/j.ydbio.2009.12.035
复制
发表时间：
2010-03-15
影响因子：
2.7
通讯作者：
Boswell RE
中科院分区：
文献类型：
--
作者：
Lewandowski JP;Sheehan KB;Bennett PE Jr;Boswell RE
在果蝇卵子发生过程中，生殖系干细胞分裂，形成一个由16个相互连接的细胞组成的包囊。一个细胞进入卵子生成途径，其余15个细胞分化为哺育细胞。尽管卵母细胞分化因子在单个细胞内的定向运输和定位对于卵母细胞的选择、维持和分化是必不可少的，但调控这些事件的机制却鲜为人知。Mago Nashi和Tsunagi/Y14是外显子连接复合体(由剪接RNA组装的多蛋白复合体)的核心成分，对于将卵母细胞的命运限制在单个细胞和Oskar mRNA的定位是必不可少的。在这里，我们提供了Mago Nashi和Tsunagi/Y14与RANSHI形成卵源复合体的证据，RANSHI是一种含有锌指相关结构域和锌指结构域的蛋白质。对冉氏卵母细胞的遗传分析表明：(1)16-细胞包囊形成，(2)两个细胞保留联会复合体，(3)所有细胞都有内复制的DNA(如在哺乳细胞中所观察到的)，(4)卵母细胞特有的细胞质标记在单个细胞内积累并持续存在，但不定位于假定的卵母细胞的后极。我们的结果表明，冉石与外显子连接复合体相互作用，将卵母细胞分化所必需的成分定位在假定卵母细胞的后极。
During Drosophila melanogaster oogenesis, a germline stem cell divides forming a cyst of 16 interconnected cells. One cell enters the oogenic pathway and the remaining 15 differentiate as nurse cells. Although directed transport and localization of oocyte differentiation factors within the single cell are indispensible for selection, maintenance, and differentiation of the oocyte, the mechanisms regulating these events are poorly understood. Mago Nashi and Tsunagi/Y14, core components of the exon junction complex (a multiprotein complex assembled on spliced RNAs), are essential for restricting oocyte fate to a single cell and for localization of oskar mRNA. Here we provide evidence that Mago Nashi and Tsunagi/Y14 form an oogenic complex with Ranshi, a protein with a zinc finger-associated domain and zinc finger domains. Genetic analyses of ranshi reveal that (1) 16-cell cysts are formed, (2) two cells retain synaptonemal complexes, (3) all cells have endoreplicated DNA (as observed in nurse cells), and (4) oocyte-specific cytoplasmic markers accumulate and persist within a single cell but are not localized within the posterior pole of the presumptive oocyte. Our results indicate that Ranshi interacts with the exon junction complex to localize components essential for oocyte differentiation within the posterior pole of the presumptive oocyte.
登录
查看更多内容
影响因子：
2.6
作者：
de Nooij, JC;Graber, KH;Hariharan, IK
通讯作者：
Hariharan, IK
影响因子：
10.5
作者：
Ghabrial, A;Ray, RP;Schüpbach, T
通讯作者：
Schüpbach, T
影响因子：
7.7
作者：
Chung, HR;Schäfer, U;Böhm, S
通讯作者：
Böhm, S
影响因子：
64.8
作者：
Andersen, JS;Wilkinson, CJ;Mann, M
通讯作者：
Mann, M
影响因子：
9.2
作者：
Dostie, J;Dreyfuss, G
通讯作者：
Dreyfuss, G