Serum antibodies to West Nile virus in naturally exposed and vaccinated horses.
Serum antibodies to West Nile virus in naturally exposed and vaccinated horses.
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天然暴露和接种的马中西尼罗河病毒的血清抗体。
DOI：
10.1099/jmm.0.47849-0
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发表时间：
2008-09
影响因子：
3
通讯作者：
Koski, Raymond A.
中科院分区：
文献类型：
--
作者：
Magnarelli, Louis A.;Bushmich, Sandra L.;Anderson, John F.;Ledizet, Michel;Koski, Raymond A.
在美国康涅狄格州和纽约州，使用多价酶联免疫吸附试验（ELISA）和空斑减少中和试验（PRNT）来测量自然接触或接种过西尼罗河病毒（WNV）黄病毒疫苗的马的血清抗体。依靠PRNT作为“金标准”，主要目的是验证含有重组WNV包膜蛋白抗原的改良ELISA。通过检测患有其他未确诊疾病的马的血清来评估特异性也很重要。后一项研究的血清是在1995 - 1996年期间从43匹私有马中获得的。ELISA和PRNT分析证实，23匹自然暴露马的血清中有21匹（91%）存在WNV抗体，20名接种受试者中有85%存在WNV抗体;两个研究组的总体结果高度一致（91%一致）。自然接触和免疫的马的体液反应相似。这两种血清学检测都有助于确认过去的西尼罗河病毒感染，但没有证据表明患有未确诊疾病的马在1999年爆发之前暴露于西尼罗河病毒。
A polyvalent enzyme-linked immunosorbent assay (ELISA) and plaque reduction neutralization tests (PRNT) were used to measure serum antibodies to the West Nile virus (WNV) in horses naturally exposed to or vaccinated against this flavivirus in Connecticut and New York State, USA. Relying on a PRNT as a “gold standard”, the main objective was to validate a modified ELISA containing a recombinant WNV envelope protein antigen. It was also important to assess specificity by testing sera from horses that had other, undiagnosed illnesses. Sera for the latter study were obtained from 43 privately owned horses during 1995−1996. Analyses by an ELISA and PRNT confirmed the presence of WNV antibodies in 21 (91%) of 23 sera from naturally exposed horses and in 85% of the 20 vaccinated subjects; overall results for both study groups were highly concordant (91% agreement). Humoral responses of naturally exposed and immunized horses were similar. Both serologic tests were useful in confirming past infections of the WNV, but there was no evidence that horses with undiagnosed illnesses were exposed to the WNV prior to the 1999 outbreak.
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DOI：
10.4269/ajtmh.2000.63.36
发表时间：
2000-07-01
影响因子：
3.3
作者：
Ebel, GD;Campbell, EN;Telford, SR
通讯作者：
Telford, SR
影响因子：
5.5
作者：
Ledizet, M;Kar, K;Koski, RA
通讯作者：
Koski, RA
DOI：
10.4269/ajtmh.1977.26.547
发表时间：
1977-01-01
影响因子：
3.3
作者：
MAGNARELLI, LA
通讯作者：
MAGNARELLI, LA
DOI：
10.2460/javma.2000.217.1045
发表时间：
2000-10-01
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Magnarelli, LA;Ijdo, JW;Fikrig, E
通讯作者：
Fikrig, E
影响因子：
2.1
作者：
Balenghien, T.;Fouque, F.;Bicout, D. J.
通讯作者：
Bicout, D. J.