Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5‐HT1A agonist, S100b, and corticosteroid receptor agonists
Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5‐HT1A agonist, S100b, and corticosteroid receptor agonists
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5-HT1A 激动剂、S100b 和皮质类固醇受体激动剂增强大鼠海马培养物中的突触素免疫反应性
作者：
M. Nishi;P. Whitaker;E. Azmitia
5-羟色胺（5-HT）已被证明在发育和成人可塑性过程中调节大脑成熟。整个动物中的这种效应可能是由于5-HT 1A受体的激活以及S100 b和皮质酮的相应增加。突触体素是突触囊泡膜的一种整合蛋白，与突触密度和神经递质释放相关，成年大鼠皮质和海马中5-HT耗竭会降低突触体素。注射5-HT 1A激动剂或地塞米松可以逆转突触素免疫反应性（IR）的丧失。在这项研究中，我们使用突触素-IR的形态计量学分析来研究5-HT 1A激动剂伊沙匹隆和神经元延伸因子S100 b对在无血清和类固醇培养基中生长的海马神经元的影响。这两种化合物在先前确定为高度特异性的剂量下增加突触体蛋白IR。Ipsapirone（10−9 M）对神经元细胞体染色更有效，S100 b（10 ng/ml）对增加神经元突起上突触素IR静脉曲张的数量更有效。此外，在先前确定的特定剂量下，两种类型的皮质类固醇受体激动剂Ru 28362（10−8 M）和醛固酮（10−9 M）与对照组相比，在细胞体染色和静脉曲张数量方面均产生较小的增加。这些分化因子对突触素IR表达的影响表明，大脑中存在多个产生和维持突触前元件的调节位点。© 1996 Wiley利斯公司
Serotonin (5‐HT) has been shown to modulate brain maturation during development and adult plasticity. This effect in the whole animal may be due to activation of 5‐HT1A receptors and a corresponding increases in S100b and corticosterone. Synaptophysin, an integral protein of the synaptic vesicle membrane that correlates with synaptic density and neurotransmitter release, is reduced by depletion of 5‐HT in the cortex and hippocampus of the adult rat. Injections of a 5‐HT1A agonist or dexamethasone can reverse the loss of synaptophysin immunoreactivity (IR). In this study we used morphometric analysis of synaptophysin‐IR to study the effects of the 5‐HT1A agonist, ipsapirone, and the neuronal extension factor, S100b on hippocampal neurons grown in a serum and steroid free media. Both compounds increased the synaptophysin‐IR at doses previously established to be highly specific. Ipsapirone (10−9 M) was more effective on neuronal cell bodies staining and S100b (10 ng/ml) was more effective in increasing the number of synaptophysin‐IR varicosities on neuronal processes. In addition both types of corticosteroid receptor agonists, at previously established specific doses, Ru28362 (10−8 M) and aldosterone (10−9 M) produced smaller increases compared to control groups in both the cell body staining and the number of varicosities. The effect of these differentiating factors on the expression of synaptophysin‐IR suggests multiple regulation sites for producing and maintaining pre‐synaptic elements in the brain. © 1996 Wiley‐Liss, Inc.
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DOI：
--
发表时间：
1991
期刊：
The American journal of pathology
影响因子：
--
作者：
E. Masliah;R. Terry;Michael;Alford;R. DeTeresa;L. Hansen
通讯作者：
E. Masliah;R. Terry;Michael;Alford;R. DeTeresa;L. Hansen
影响因子：
5
作者：
M. Owens;E. Edwards;C. Nemeroff
通讯作者：
M. Owens;E. Edwards;C. Nemeroff
S100 beta 刺激神经胶质细胞和神经元细胞中的钙流动。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Barger,SW;VanEldik,LJ
通讯作者：
VanEldik,LJ
影响因子：
56.9
作者：
ANDRADE, R;MALENKA, RC;NICOLL, RA
通讯作者：
NICOLL, RA