Biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro
Biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro
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表皮生长因子对离体大鼠颅骨细胞骨结节形成的双相作用
DOI：
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发表时间：
1987
影响因子：
6.2
通讯作者：
J. Aubin
中科院分区：
文献类型：
--
作者：
M. Antosz;C. G. Bellows;J. Aubin
从胎鼠颅骨（RC）中分离的成骨细胞样细胞在体外形成三维结节，具有骨的形态学和免疫组织化学特征。在21天的培养过程中，当浓度在10 - 12至10 - 7M之间时，EGF引起了与剂量相关的结节形成抑制，并降低了矿化结节的比例。在同样的条件下，培养物中的细胞增殖和饱和密度都增加了，这表明减少的结节形成不是一般细胞毒性的结果。EGF的抑制浓度引起细胞形状的可逆改变，尽管蛋白质合成的变化未被检测到。吲哚美辛（5 × 10−7 M）既不影响结节形成，也不影响EGF对其的抑制作用。用10 - 8 M EGF对培养物进行不同时间的预处理，然后将其去除并在补充培养基中继续培养21天，与单独在补充培养基中形成的骨结节数量相比，短暂暴露于EGF（4小时至48小时）形成的骨结节数量增加，而暴露于EGF 4天或更长时间则减少了形成的结节数量。这些数据表明，EGF可以增加或减少体外RC细胞的成骨潜能，这取决于暴露于该因子的持续时间。
Osteoblast‐like cells isolated from fetal rat calvaria (RC) form three‐dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. When administered continually over 21 days of culture, EGF caused a dose‐related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10−12 to 10−7M. The same conditions caused an increase in both cell proliferation and saturation density in the cultures, suggesting that decreased nodule formation was not the result of general cell toxicity. Inhibitory concentrations of EGF caused a reversible alteration in cell shape, although changes in protein synthesis were not detectable. Indomethacin (5 × 10−7 M) did not affect either nodule formation or its inhibition by EGF. When cultures were pretreated for various time periods with 10−8 M EGF followed by its removal and continued culture in supplemented medium for 21 days, the number of bone nodules formed with brief exposures to EGF (4 hr up to 48 hr) was increased compared to the numbers formed in supplemented medium alone, whereas exposures to EGF of 4 days or longer decreased the number of nodules formed. These data indicate that EGF can either increase or decrease the osteogenic potential of RC cells in vitro depending on the duration of exposure to the factor.
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影响因子：
9.8
作者：
CANALIS, E
通讯作者：
CANALIS, E
α 和 β 人类转化生长因子刺激培养小鼠颅骨中前列腺素的产生和骨吸收。
DOI：
10.1073/pnas.82.13.4535
发表时间：
1985
影响因子：
11.1
作者：
TashjianJr,AH;Voelkel,EF;Lazzaro,M;Singer,FR;Roberts,AB;Derynck,R;Winkler,ME;Levine,L
通讯作者：
Levine,L
表皮生长因子对人成纤维细胞合成活性的影响。
DOI：
10.1016/0304-4165(80)90080-x
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Huey,J;Narayanan,AS;Jones,K;Page,RC
通讯作者：
Page,RC
合成转化生长因子-α 刺激体外骨吸收。
DOI：
10.1126/science.3859011
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ibbotson,KJ;Twardzik,DR;D'Souza,SM;Hargreaves,WR;Todaro,GJ;Mundy,GR
通讯作者：
Mundy,GR