Isolation and chromosomal localization of new MITE-like sequences from Secale

Isolation and chromosomal localization of new MITE-like sequences from Secale
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黑麦新 MITE 样序列的分离和染色体定位

DOI:
10.2478/s11756-011-0139-6
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发表时间:
2012-01
期刊:
影响因子:
1.5
通讯作者:
Yang, Zujun
Yang, Zujun
中科院分区:
生物学4区
文献类型:
--
作者:
Hu, Lijun;Zeng, Zixian;Liu, Cheng;Li, Guangrong;Yang, Zujun

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利用小麦微卫星引物Xgwm614,构建了一个在普通小麦背景下检测黑麦染色质存在的种特异性DNA序列(标记)。从非洲黑麦和非洲小麦两倍体中获得了一条416bp的黑麦特异性DNA扩增片段(pSa614416)。引物Xgwm614在中国所有春-帝王黑麦附加系1R ~ 7R中也产生了特异条带。序列分析表明,pSa614416与一个微型倒转座元件(microrated inverted transposable element,简称MITE)偷渡者样元件高度同源。荧光原位杂交结果表明,pSa614416的信号分布在所有的谷类小麦中。但在7条黑麦基因组染色体上信号强度不平衡。该重复元件可能作为黑麦种质向小麦基因组渗入的分子标记。
A species-specific DNA sequence (marker) that can detect the presence of Secale cereale chromatin in common wheat background was developed by using wheat microsatellite primer Xgwm614. One rye-specific DNA amplified fragment of 416bp (pSa614416) was obtained from Secale africanum and a wheat — S. africanum amphiploid. The primer Xgwm614 also gave rise to specific bands in all Chinese Spring-Imperial rye addition lines 1R to 7R. Sequence analysis revealed that pSa614416 was strongly homologous to a miniature inverted transposable element (MITE) stowaway-like element. Results of fluorescence in situ hybridization showed that the signal of pSa614416 was distributed along all S. cereale. cv Jingzhou chromosomes, but the signal strengths were unbalanced on the seven rye genome chromosomes. This repetitive element may be useful as a molecular marker for the introgression of rye germplasm into the wheat genome.
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