Comparison of traditional intranasal and aerosol inhalation inoculation of mice with influenza A viruses.
Comparison of traditional intranasal and aerosol inhalation inoculation of mice with influenza A viruses.
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DOI：
10.1016/j.virol.2015.02.041
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发表时间：
2015-07
期刊：
影响因子：
3.7
通讯作者：
Tumpey TM
中科院分区：
文献类型：
--
作者：
Belser JA;Gustin KM;Katz JM;Maines TR;Tumpey TM
鼻内滴注流感病毒混悬液（IN）是用流感病毒感染小型哺乳动物模型的最常用方法，但并不反映自然暴露途径。相比之下，通过雾化吸入（AR）接种更接近于人类暴露于流感病毒。在小鼠中进行的研究在通过这些途径接种的病毒诱导的毒力方面产生了相互矛盾的结果，并且没有控制潜在的株特异性差异，也没有检查具有大流行潜力的当代流感病毒和禽流感病毒。我们使用全身AR接种方法在小鼠模型中比较高致病性H5N1、低致病性H7N9和2009 H1N1病毒与传统IN接种的感染性和疾病进展。使用任一接种途径检查的所有病毒均观察到发病率和死亡率水平大体相当，表明IN和AR递送均适用于研究流感病毒致病性的鼠研究。
Intranasal instillation of virus in a liquid suspension (IN) is the most frequently employed method to inoculate small mammalian models with influenza virus, but does not reflect a natural route of exposure. In contrast, inoculation via aerosol inhalation (AR) more closely resembles human exposure to influenza virus. Studies in mice have yielded conflicting results regarding virulence induced by virus inoculated by these routes, and have not controlled for potential strain-specific differences, or examined contemporary influenza viruses and avian viruses with pandemic potential. We used a whole-body AR inoculation method to compare infectivity and disease progression of a highly pathogenic H5N1, a low pathogenic H7N9, and a 2009 H1N1 virus with traditional IN inoculation in the mouse model. Generally comparable levels of morbidity and mortality were observed with all viruses examined using either inoculation route, indicating that both IN and AR delivery are appropriate for murine studies investigating influenza virus pathogenicity.
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