Hen uterine gene expression profiling during eggshell formation reveals putative proteins involved in the supply of minerals or in the shell mineralization process.
Hen uterine gene expression profiling during eggshell formation reveals putative proteins involved in the supply of minerals or in the shell mineralization process.
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DOI：
10.1186/1471-2164-15-220
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发表时间：
2014-03-21
期刊：
影响因子：
4.4
通讯作者：
Gautron J
中科院分区：
文献类型：
--
作者：
Brionne A;Nys Y;Hennequet-Antier C;Gautron J
蛋壳是一种天然的机械屏障，可以保护鸡蛋成分免受物理损伤和微生物的渗透。它的完整性和强度对于胚胎的发育或确保消费者的餐桌鸡蛋没有病原体至关重要。本研究比较了蛋鸡子宫在有无壳钙化的情况下的整体基因表达，以确定参与矿物质供应和/或壳层生物矿化过程的基因产物。微阵列被用来鉴定在贝壳钙化过程中过度表达的302个基因。GO术语丰富提供了对过度表达基因功能的全球解释，并揭示了大多数过度表达的蛋白质与生殖功能有关。我们的分析确定了16种编码与矿物质供应有关的蛋白质的基因产物，并允许更新描述蛋壳钙化过程中子宫离子转运蛋白的一般模型。还建立了可能分泌到子宫液中的57种蛋白质在矿化过程中的活性。根据它们的潜在功能(生物矿化、蛋白多糖、分子伴侣、抗菌剂和蛋白酶/抗蛋白酶)进行了分类。我们的研究提供了壳层矿化时过度表达的基因和相应蛋白质的详细描述。其中一些参与矿物质供应并影响蛋壳结构以保护蛋壳内容物的蛋白质是潜在的有益于蛋壳品质遗传改良的生物标记。
The chicken eggshell is a natural mechanical barrier to protect egg components from physical damage and microbial penetration. Its integrity and strength is critical for the development of the embryo or to ensure for consumers a table egg free of pathogens. This study compared global gene expression in laying hen uterus in the presence or absence of shell calcification in order to characterize gene products involved in the supply of minerals and / or the shell biomineralization process. Microarrays were used to identify a repertoire of 302 over-expressed genes during shell calcification. GO terms enrichment was performed to provide a global interpretation of the functions of the over-expressed genes, and revealed that the most over-represented proteins are related to reproductive functions. Our analysis identified 16 gene products encoding proteins involved in mineral supply, and allowed updating of the general model describing uterine ion transporters during eggshell calcification. A list of 57 proteins potentially secreted into the uterine fluid to be active in the mineralization process was also established. They were classified according to their potential functions (biomineralization, proteoglycans, molecular chaperone, antimicrobials and proteases/antiproteases). Our study provides detailed descriptions of genes and corresponding proteins over-expressed when the shell is mineralizing. Some of these proteins involved in the supply of minerals and influencing the shell fabric to protect the egg contents are potentially useful biological markers for the genetic improvement of eggshell quality.
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影响因子：
14.9
作者：
Benson DA;Karsch-Mizrachi I;Lipman DJ;Ostell J;Sayers EW
通讯作者：
Sayers EW
影响因子：
14.9
作者：
Briesemeister S;Rahnenführer J;Kohlbacher O
通讯作者：
Kohlbacher O
DOI：
10.1186/1477-7827-7-27
发表时间：
2009-04-05
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Gao L;Cong B;Zhang L;Ni X
通讯作者：
Ni X
DOI：
10.1093/bioinformatics/btq115
发表时间：
2010-05-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Briesemeister S;Rahnenführer J;Kohlbacher O
通讯作者：
Kohlbacher O
影响因子：
3.6
作者：
EASTIN, WC;SPAZIANI, E
通讯作者：
SPAZIANI, E