Agents that activate protein kinase C reduce acetylcholine sensitivity in cultured myotubes.
Agents that activate protein kinase C reduce acetylcholine sensitivity in cultured myotubes.
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激活蛋白激酶C的药物降低了培养的肌管中的乙酰胆碱敏感性。
DOI：
10.1083/jcb.100.4.1339
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发表时间：
1985-04
影响因子：
7.8
通讯作者：
ZANI, BM
中科院分区：
文献类型：
--
作者：
EUSEBI, F;MOLINARO, M;ZANI, BM
我们已经研究了乙酰胆碱（ACh）引起的电位或电流在电流或电压钳位培养的肌管暴露于12-O-十四烷酰基-佛波醇-13-乙酸酯（TPA），一种有效的肿瘤促进剂，激活蛋白激酶C。虽然这种药物对膜静息电位或电阻几乎没有作用，但在3-4-d龄鸡肌管上诱导ACh敏感性可逆性降低。TPA的递质作用的抑郁症被扩展到7-8-d小鼠肌管，只有当他们与磷脂酰丝氨酸治疗。甘油二油酸酯对肌管的作用与TPA相似，但效力降低。我们的结论是，蛋白激酶C的激活可能参与的ACh受体的能力，以响应递质刺激。
We have examined acetylcholine (ACh)-elicited potentials or currents in current- or voltage-clamped cultured myotubes exposed to 12-O- tetradecanoyl-phorbol-13-acetate (TPA), a potent tumor promoter that activates protein kinase C. Although this agent had little action on either membrane resting potential or electrical resistance, a reversible decrease in ACh sensitivity was induced on 3-4-d-old chick myotubes. Depression of transmitter action by TPA was extended to 7-8-d mouse myotubes only when they were treated with phosphatidylserine. Glyceryl dioleate had effects on myotubes similar to those of TPA but with a reduced efficacy. We conclude that the activation of protein kinase C might be involved with the capacity of ACh receptors to respond to transmitter stimulation.
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DOI：
10.1016/0045-6039(76)90015-4
发表时间：
1976-01-01
期刊：
CELL DIFFERENTIATION
影响因子：
--
作者：
ADAMO, S;ZANI, B;MOLINARO, M
通讯作者：
MOLINARO, M
影响因子：
5.5
作者：
TAKEUCHI, N
通讯作者：
TAKEUCHI, N
影响因子：
3.8
作者：
RITCHIE, AK;FAMBROUGH, DM
通讯作者：
FAMBROUGH, DM
影响因子：
3.4
作者：
COSSU, G;EUSEBI, F;MOLINARO, M
通讯作者：
MOLINARO, M
影响因子：
2.7
作者：
CROOP, J;DUBYAK, G;HOLTZER, H
通讯作者：
HOLTZER, H