Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform.
Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform.
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DOI：
10.1186/s12951-022-01511-9
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发表时间：
2022-06-28
影响因子：
10.2
通讯作者：
Khoshfetrat, Ali Baradar
中科院分区：
文献类型：
--
作者：
Hassani, Ayla;Avci, Cigir Biray;Kerdar, Sajed Nazif;Amini, Hassan;Amini, Meisam;Ahmadi, Mahdi;Sakai, Shinji;Bagca, Bakiye Goker;Ozates, Neslihan Pinar;Rahbarghazi, Reza;Khoshfetrat, Ali Baradar
关键词：
基于有机/无机复合材料的水凝胶一直是工程化骨构建材料的研究热点。建立一个直截了当的3D微环境对于维持细胞间的相互作用和细胞功能，导致适当的再生至关重要。采用离子交联剂Ca~(2+)、Ba2+和Sr~(2+)制备了海藻酸盐-纳米羟基磷灰石-胶原(Alg-nHA-Col)微球，并在体外和体内条件下检测了成骨细胞的成骨特性。水凝胶的物理化学性质表明，与其他基团相比，以Sr2+为交联剂的微球具有较小的溶胀、增强的稳定性和机械强度。人MG-63成骨细胞在Sr2+交联微球内的成骨活性和成骨能力增强，矿化和骨形成相关蛋白增加。对WNT(Wingless-Related Integration Site)信号通路的聚合酶链式反应(Polymerase Chain Reaction，PCR)阵列分析表明，Sr2+交联微球适当地诱导人成骨细胞的多种信号转导通路，从而促进成骨活性和动态生长。将Sr2+交联微球与大鼠成骨细胞复合移植到大鼠颅骨临界大小缺损区，通过显微CT和组织学检查分析其促进骨形成的作用。Sr2+交联Alg-NHA-Col水凝胶可促进成骨细胞的功能和动态生长。网上版载有补充材料，可在10.1186/s12951-022-01511-9查阅。
Hydrogels based on organic/inorganic composites have been at the center of attention for the fabrication of engineered bone constructs. The establishment of a straightforward 3D microenvironment is critical to maintaining cell-to-cell interaction and cellular function, leading to appropriate regeneration. Ionic cross-linkers, Ca2+, Ba2+, and Sr2+, were used for the fabrication of Alginate-Nanohydroxyapatite-Collagen (Alg-nHA-Col) microspheres, and osteogenic properties of human osteoblasts were examined in in vitro and in vivo conditions after 21 days. Physicochemical properties of hydrogels illustrated that microspheres cross-linked with Sr2+ had reduced swelling, enhanced stability, and mechanical strength, as compared to the other groups. Human MG-63 osteoblasts inside Sr2+ cross-linked microspheres exhibited enhanced viability and osteogenic capacity indicated by mineralization and the increase of relevant proteins related to bone formation. PCR (Polymerase Chain Reaction) array analysis of the Wnt (Wingless-related integration site) signaling pathway revealed that Sr2+ cross-linked microspheres appropriately induced various signaling transduction pathways in human osteoblasts leading to osteogenic activity and dynamic growth. Transplantation of Sr2+ cross-linked microspheres with rat osteoblasts into cranium with critical size defect in the rat model accelerated bone formation analyzed with micro-CT and histological examination. Sr2+ cross-linked Alg-nHA-Col hydrogel can promote functionality and dynamic growth of osteoblasts. The online version contains supplementary material available at 10.1186/s12951-022-01511-9.
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影响因子：
5.5
作者：
Cheshmedzhieva D;Ilieva S;Permyakov EA;Permyakov SE;Dudev T
通讯作者：
Dudev T
影响因子：
11.4
作者：
Bianco S;Mancardi D;Merlino A;Bussolati B;Munaron L
通讯作者：
Munaron L
DOI：
10.1016/j.msec.2019.03.040
发表时间：
2019-07-01
影响因子：
7.9
作者：
Bi, Yong-guang;Lin, Zi-ting;Deng, Shi-ting
通讯作者：
Deng, Shi-ting
影响因子：
14
作者：
Ehara, A;Ogata, K;Umakoshi, Y
通讯作者：
Umakoshi, Y
影响因子：
18.9
作者：
Geng Z;Ji L;Li Z;Wang J;He H;Cui Z;Yang X;Liu C
通讯作者：
Liu C