Nano-needle strontium-substituted apatite coating enhances osteoporotic osseointegration through promoting osteogenesis and inhibiting osteoclastogenesis.
Nano-needle strontium-substituted apatite coating enhances osteoporotic osseointegration through promoting osteogenesis and inhibiting osteoclastogenesis.
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DOI：
10.1016/j.bioactmat.2020.09.024
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发表时间：
2021-04
影响因子：
18.9
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Geng Z;Ji L;Li Z;Wang J;He H;Cui Z;Yang X;Liu C
种植体松动仍然是骨质疏松患者面临的主要临床挑战。这是因为在骨质疏松的情况下，破骨细胞的骨吸收率高于成骨细胞的骨形成率，从而导致骨修复不良。锶（Sr）已被广泛认为是一种抗骨质疏松元素。在这项研究中，我们在不同的酸性条件下通过电化学沉积制备了一系列磷灰石和锶取代的磷灰石涂层。结果表明，Ca和Sr表现出不同的矿化行为。 Ca的主要矿化产物为CaHPO4·2H2O和Ca3(PO4)2,随着pH值的升高,其结构由多孔状变为球形。 Sr的主要矿化产物为SrHPO4和Sr5(PO4)3OH，随着pH值的升高，其结构由片状变为针状。体外实验表明，在存在Sr的高pH条件下制备的涂层有利于MSCs的粘附、铺展、增殖和成骨分化。此外，Sr取代的磷灰石涂层可以明显抑制破骨细胞的分化和融合。此外，体内研究表明，纳米针状锶取代磷灰石涂层可以抑制破骨细胞活性，改善新骨形成，并增强骨-种植体整合。这项研究为种植体涂层设计提供了新的理论指导，所制造的锶替代涂层可能对骨质疏松患者有潜在的应用。 Ca2+和Sr2+在酸性环境中表现出不同的矿化行为。在高 pH 条件下制备的磷灰石有利于 MSC 的生长。 Sr取代磷灰石表现出优异的抗破骨细胞活性能力。锶取代的磷灰石促进成骨、骨生长和骨整合。
Implant loosening remains a major clinical challenge for osteoporotic patients. This is because osteoclastic bone resorption rate is higher than osteoblastic bone formation rate in the case of osteoporosis, which results in poor bone repair. Strontium (Sr) has been widely accepted as an anti-osteoporosis element. In this study, we fabricated a series of apatite and Sr-substituted apatite coatings via electrochemical deposition under different acidic conditions. The results showed that Ca and Sr exhibited different mineralization behaviors. The main mineralization products for Ca were CaHPO4·2H2O and Ca3(PO4)2 with the structure changed from porous to spherical as the pH values increased. The main mineralization products for Sr were SrHPO4 and Sr5(PO4)3OH with the structure changed from flake to needle as the pH values increased. The in vitro experiment demonstrated that coatings fabricated at high pH condition with the presence of Sr were favorable to MSCs adhesion, spreading, proliferation, and osteogenic differentiation. In addition, Sr-substituted apatite coatings could evidently inhibit osteoclast differentiation and fusion. Moreover, the in vivo study indicated that nano-needle like Sr-substituted apatite coating could suppress osteoclastic activity, improve new bone formation, and enhance bone-implant integration. This study provided a new theoretical guidance for implant coating design and the fabricated Sr-substituted coating might have potential applications for osteoporotic patients. Ca2+ and Sr2+ showed different mineralization behaviors in acidic environments. Apatites fabricated at high pH conditions were beneficial to MSCs growth. Sr-substituted apatite exhibited superior anti-osteoclast activity ability. Sr-substituted apatite facilitated osteogenesis, bone growth, and osseointegration.
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影响因子：
9.7
作者：
Luo, Xiaoman;Barbieri, Davide;Bruijn, Joost D.
通讯作者：
Bruijn, Joost D.
DOI：
10.1016/j.msec.2019.109933
发表时间：
2019-11-01
影响因子：
7.9
作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
16.6
作者：
Cui, Yidan;Hameed, Feroz M.;Yang, Bo;Lee, Kyunghee;Pan, Catherine Qiurong;Park, Sungsu;Sheetz, Michael
通讯作者：
Sheetz, Michael
影响因子：
168.9
作者：
Glyn-Jones, S.;Palmer, A. J. R.;Carr, A. J.
通讯作者：
Carr, A. J.