The mouse viral outgrowth assay: avatars for the detection of HIV-1 reservoirs.

The mouse viral outgrowth assay: avatars for the detection of HIV-1 reservoirs.
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DOI:
10.1186/s12977-017-0376-z
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发表时间:
2017-11-21
期刊:
影响因子:
3.3
通讯作者:
Blankson JN
Blankson JN
中科院分区:
医学2区
文献类型:
--
作者:
Metcalf Pate KA;Blankson JN

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需要灵敏的检测方法来检测接受抑制性联合抗逆转录病毒治疗方案的HIV-1感染受试者中的残留病毒储库,以确定根除策略是否有效。最近开发了小鼠病毒生长测定法,并且具有比传统的体外定量病毒生长测定法更灵敏的潜力。在这篇文章中,我们描述了这些检测和审查几项研究,用它们来衡量潜在的水库。
Sensitive assays are needed for the detection of residual viral reservoirs in HIV-1-infected subjects on suppressive combination antiretroviral therapy regimens to determine whether eradication strategies are effective. Mouse viral outgrowth assays have recently been developed and have the potential to be more sensitive than traditional in vitro quantitative viral outgrowth assays. In this article we describe these assays and review several studies that have used them to measure the latent reservoir.
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