Critical factors affecting cell encapsulation in superporous hydrogels.
Critical factors affecting cell encapsulation in superporous hydrogels.
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DOI：
10.1088/1748-6041/7/2/024108
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发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
Gemeinhart RA
中科院分区：
文献类型：
--
作者：
Desai ES;Tang MY;Ross AE;Gemeinhart RA
我们最近的研究表明，当将细胞植入预制SPH支架的孔隙中时，超孔水凝胶支架可以促进干细胞的长期活力和细胞驱动的矿化。本研究进一步探讨了SPH水凝胶基质在制备过程中细胞包封的可能性。系统地检查了SPH制造过程中使用的每种化学成分和制造过程的每个步骤对细胞活力的影响。过硫酸铵（引发剂）和碳酸氢钠（气体生成化合物）是制备SPH所需浓度下对被封装细胞具有显著毒性的两种成分。过硫酸铵和碳酸氢钠溶液作用10分钟后，细胞存活率分别为55.7±19.3%和88.8±9.4%。此外，通过添加碳酸氢钠改变溶液pH对被包被细胞有明显的毒性，细胞存活率仅为50.3±2.5%。尽管化学成分和制备方法具有毒性，但包封后48和72小时，细胞在SPH内的总存活率分别为81.2±6.8和67.0±0.9%。这种细胞包封方法有望在体外和体内作为水凝胶基质包封和细胞在孔隙内播种的支架材料使用。
We recently showed that superporous hydrogel scaffolds promote long-term stem cell viability and cell driven mineralization when cells were seeded within the pores of pre-fabricated SPH scaffolds. The possibility of cell encapsulation within SPH hydrogel matrix during its fabrication was further explored in this study. The impact of each chemical component used in the SPH fabrication and each step of the fabrication process on cell viability was systematically examined. Ammonium persulfate, an initiator, and sodium bicarbonate, the gas-generating compound, were the two components having significant toxicity toward encapsulated cells at the concentrations necessary for SPH fabrication. Cell survival rates were 55.7±19.3% and 88.8±9.4% after 10 minute exposure to ammonium persulfate and sodium bicarbonate solutions, respectively. In addition, solution pH change via the addition of sodium bicarbonate had significant toxicity toward encapsulated cells with cell survival of only 50.3±2.5%. Despite toxicity of chemical components and the SPH fabrication method, cells still exhibited significant overall survival rates within SPHs of 81.2±6.8 and 67.0±0.9%, respectively, 48 and 72 hours after encapsulation. This method of cell encapsulation holds promise for use in vitro an in vivo as a scaffold material for both hydrogel matrix encapsulation and cell seeding within the pores.
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影响因子：
9
作者：
Hwang CM;Sant S;Masaeli M;Kachouie NN;Zamanian B;Lee SH;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
14
作者：
Mann, BK;Gobin, AS;West, JL
通讯作者：
West, JL
影响因子：
--
作者：
Johnson, Peter C.;Mikos, Antonios G.;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
4.8
作者：
Clark, Paul A.;Moioli, Eduardo K.;Mao, Jeremy J.
通讯作者：
Mao, Jeremy J.
DOI：
10.1073/pnas.0737381100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Lutolf, MP;Lauer-Fields, JL;Hubbell, JA
通讯作者：
Hubbell, JA